{"created":"2023-05-15T16:48:59.868342+00:00","id":26248,"links":{},"metadata":{"_buckets":{"deposit":"187c9b0b-c91d-4b2e-ab14-b5fadb32fc35"},"_deposit":{"created_by":2,"id":"26248","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"26248"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026248","sets":["66:67"]},"author_link":["111965","111966","111969","111968","111967"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-12-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"21146","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cellular metabolism is directly or indirectly associated with various cellular processes by producing a variety of metabolites. Metabolic alterations may cause adverse effects on cell viability. However, some alterations potentiate the rescue of the malfunction \nof the cell system. Here, we found that the alteration of glucose metabolism suppressed genome instability caused by the impairment \nof chromatin structure. Deletion of the TDH2 gene, which encodes glyceraldehyde 3-phospho dehydrogenase and is essential for \nglycolysis/gluconeogenesis, partially suppressed DNA damage sensitivity due to chromatin structure, which was persistently \nacetylated histone H3 on lysine 56 in cells with deletions of both HST3 and HST4, encoding NAD+-dependent deacetylases. \ntdh2 deletion also restored the short replicative lifespan of cells with deletion of sir2, another NAD+-dependent deacetylase, \nby suppressing intrachromosomal recombination in rDNA repeats increased by the unacetylated histone H4 on lysine 16. tdh2 deletion \nalso suppressed recombination between direct repeats in hst3? hst4? cells by suppressing the replication fork instability that leads \nto both DNA deletions among repeats. We focused on quinolinic acid (QUIN), a metabolic intermediate in the de novo nicotinamide \nadenine dinucleotide (NAD+) synthesis pathway, which accumulated in the tdh2 deletion cells and was a candidate metabolite \nto suppress DNA replication fork instability. Deletion of QPT1, quinolinate phosphoribosyl transferase, elevated intracellular\n QUIN levels and partially suppressed the DNA damage sensitivity of hst3? hst4? cells as well as tdh2? cells. qpt1 deletion restored \nthe short replicative lifespan of sir2? cells by suppressing intrachromosomal recombination among rDNA repeats. In addition, \nqpt1 deletion could suppress replication fork slippage between direct repeats. These findings suggest a connection between glucose \nmetabolism and genomic stability.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Scientific Reports, 10(1), art.no.21146; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-020-78302-5","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020, The Author(s). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hanasaki, Miki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yaku, Keisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamauchi, Motohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagawa, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masumoto, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-24"}],"displaytype":"detail","filename":"SciRep10_21146.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SciRep10_21146.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26248/files/SciRep10_21146.pdf"},"version_id":"93bf224d-dc93-44df-bd5a-2e081f2cd37b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Deletion of the GAPDH gene contributes to genome stability in Saccharomyces cerevisiae","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Deletion of the GAPDH gene contributes to genome stability in Saccharomyces cerevisiae"}]},"item_type_id":"2","owner":"2","path":["67"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-12-22"},"publish_date":"2020-12-22","publish_status":"0","recid":"26248","relation_version_is_last":true,"title":["Deletion of the GAPDH gene contributes to genome stability in Saccharomyces cerevisiae"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:02:32.516926+00:00"}