{"created":"2023-05-15T16:49:00.910994+00:00","id":26273,"links":{},"metadata":{"_buckets":{"deposit":"7d0f53c5-0ac6-41e9-8254-3194be5eaa32"},"_deposit":{"created_by":6,"id":"26273","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26273"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026273","sets":["35:36"]},"author_link":["112301","112297","112298","112300","112299"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"163","bibliographicPageStart":"155","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"Cell Structure and Function"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The smallest arenavirus gene product, Z protein, plays critical roles in the virus life cycle. Z is the major driving force of budding and particle production because of a unique property that defines self-assembly. In addition to the roles in budding, Z also participates in the suppression of type I interferon production to evade host antiviral immunity. Therefore, Z and its assembled form are an attractive drug target for arenaviral hemorrhagic fever, such as Lassa fever. Here, we developed a biosensor that enabled the evaluation of the prototype arenavirus, lymphocytic choriomeningitis virus (LCMV), Z assembly using the principle of Förster resonance energy transfer (FRET). This FRET biosensor consisted of three tandem Z that were sandwiched between super-enhanced cyan-emitting fluorescent protein and variant of a yellow-emitting mutant of green fluorescent protein so that Z-Z intermolecular binding via the really interesting new gene finger domain increased the emission ratio. To identify novel anti-arenavirus compounds, the FRET biosensor was employed to screen the PathogenBox400 for inhibitors of Z assembly in a 96-well plate format. The assay performed well, with a Z’-factor of 0.89, and identified two compounds that decreased the emission ratio of the FRET biosensor in a dose-dependent manner. Of them, the compound, 5,6,7,8-tetrahydro-7-(benzyl)-pyrido[4',3':4,5]thieno[2,3-d] pyrimidin-2,4-diamine, was found to significantly inhibit LCMV propagation in infected cells. Thereby, the present study demonstrated that a novel FRET biosensor incorporating Z assembly built on FRET and named Zabton, was a valuable screening tool to identify anti-arenavirus compounds in the context of inhibition of Z assembly.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Cell Structure and Function, 45(2), pp.155-163; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本細胞生物学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1247/csf.20030","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2020 The Author(s). This is an open access article distributed under the terms of the Creative Commons BY (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-7196","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Japan Society for Cell Biology"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mizutani, Tatsuaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohba, Yusuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mizuta, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuda, Jiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Urata, Shuzo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-01-25"}],"displaytype":"detail","filename":"CSF45_155.pdf","filesize":[{"value":"485.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CSF45_155.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26273/files/CSF45_155.pdf"},"version_id":"f94f9212-8171-4f76-9a53-e7f39c30fed6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Anti-viral drugs","subitem_subject_scheme":"Other"},{"subitem_subject":"Arenavirus","subitem_subject_scheme":"Other"},{"subitem_subject":"Förster resonance energy transfer","subitem_subject_scheme":"Other"},{"subitem_subject":"Z protein","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An Antiviral Drug Screening Platform with a FRET Biosensor for Measurement of Arenavirus Z Assembly","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An Antiviral Drug Screening Platform with a FRET Biosensor for Measurement of Arenavirus Z Assembly"}]},"item_type_id":"2","owner":"6","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-01-22"},"publish_date":"2021-01-22","publish_status":"0","recid":"26273","relation_version_is_last":true,"title":["An Antiviral Drug Screening Platform with a FRET Biosensor for Measurement of Arenavirus Z Assembly"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T22:55:24.069133+00:00"}