{"created":"2023-05-15T16:49:02.260968+00:00","id":26306,"links":{},"metadata":{"_buckets":{"deposit":"c9a612a2-0605-4515-a0a1-3a17bf2f06ce"},"_deposit":{"created_by":6,"id":"26306","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26306"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026306","sets":["143:144"]},"author_link":["117049","117048","117047","117050"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-01-19","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"113718","bibliographicVolumeNumber":"304","bibliographic_titles":[{"bibliographic_title":"General and Comparative Endocrinology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Gonadotropin-releasing hormone (GnRH) is one of the most important neuroendocrine regulators for animal reproduction. GnRH-like peptide (GnRH-like)has recently been shown to play a critical reproductive role mainly in gametogenesis or steroidogenesis in the gonads of some molluscs, including cephalopods. However, its involvement in gonadal sex differentiation remains unknown. Here, we show the expression profile of GnRH-like in the brain of the cephalopod kisslip cuttlefish, Sepia lycidas, throughout gonadal sex differentiation, by quantitative real time RT-PCR and immunohistochemistry. We found that GnRH-like could be detected in the brain at a sexually undifferentiated stage, and its expression level significantly increased upon initiation of gonadal sex differentiation. However, no significant difference in GnRH-like expression levels was observed between sexes during gonadal sex differentiation. Additionally, we demonstrated immunoreactivity of GnRH-like in glial cells or immature neurons, which are mainly distributed in the non-reproductive related area of the cephalopod brain, suggesting the immature function of the reproductive endocrine axis during early ontogenesis. Our results demonstrate for the first time, the expression profile of GnRH-like during early ontogenesis in cephalopods.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"General and Comparative Endocrinology, 304, art.no.113718; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ygcen.2021.113718","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/                                                                                       "}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00166480","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Murata, Ryosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mushirobira, Yuji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Yoshiaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Soyano, Kiyoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-19"}],"displaytype":"detail","filename":"GCE304_113718.pdf","filesize":[{"value":"27.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"GCE304_113718.pdf ","url":"https://nagasaki-u.repo.nii.ac.jp/record/26306/files/GCE304_113718.pdf"},"version_id":"acb48156-6310-44f6-9950-cc8cbdf6b4a5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Gonadotropin-releasing hormone","subitem_subject_scheme":"Other"},{"subitem_subject":"Mollusc","subitem_subject_scheme":"Other"},{"subitem_subject":"Reproduction","subitem_subject_scheme":"Other"},{"subitem_subject":"Sex","subitem_subject_scheme":"Other"},{"subitem_subject":"Squid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression profile of GnRH-like peptide during gonadal sex differentiation in the cephalopod kisslip cuttlefish, Sepia lycidas","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression profile of GnRH-like peptide during gonadal sex differentiation in the cephalopod kisslip cuttlefish, Sepia lycidas"}]},"item_type_id":"2","owner":"6","path":["144"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-02-09"},"publish_date":"2021-02-09","publish_status":"0","recid":"26306","relation_version_is_last":true,"title":["Expression profile of GnRH-like peptide during gonadal sex differentiation in the cephalopod kisslip cuttlefish, Sepia lycidas"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:37:28.269724+00:00"}