{"created":"2023-05-15T16:49:04.434570+00:00","id":26359,"links":{},"metadata":{"_buckets":{"deposit":"4109b079-7d5a-41da-8848-304705d875f6"},"_deposit":{"created_by":6,"id":"26359","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26359"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026359","sets":["35:36"]},"author_link":["117483","117482","117480","117481","117479"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-02-05","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"603924 ","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Frontiers in Immunology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Toxoplasma gondii is a neurotropic protozoan parasite, which is linked to neurological manifestations in immunocompromised individuals as well as severe neurodevelopmental sequelae in congenital toxoplasmosis. While the complement system is the first line of host defense that plays a significant role in the prevention of parasite dissemination, Toxoplasma artfully evades complement-mediated clearance via recruiting complement regulatory proteins to their surface. On the other hand, the details of Toxoplasma and the complement system interaction in the brain parenchyma remain elusive. In this study, infection-induced changes in the mRNA levels of complement components were analyzed by quantitative PCR using a murine Toxoplasma infection model in vivo and primary glial cells in vitro. In addition to the core components C3 and C1q, anaphylatoxin C3a and C5a receptors (C3aR and C5aR1), as well as alternative complement pathway components properdin (CFP) and factor B (CFB), were significantly upregulated 2 weeks after inoculation. Two months post-infection, CFB, C3, C3aR, and C5aR1 expression remained higher than in controls, while CFP upregulation was transient. Furthermore, Toxoplasma infection induced significant increase in CFP, CFB, C3, and C5aR1 in mixed glial culture, which was abrogated when microglial activation was inhibited by pre-treatment with minocycline. This study sheds new light on the roles for the complement system in the brain parenchyma during Toxoplasma infection, which may lead to the development of novel therapeutic approaches to Toxoplasma infection-induced neurological disorders.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Frontiers in Immunology, 11, art.no.603924; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers Media S.A."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3389/fimmu.2020.603924","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 Shinjyo, Hikosaka, Kido, Yoshida and Norose. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that theoriginal publication in this journal is cited, in accordance with accepted academicpractice. No use, distribution or reproduction is permitted which does not comply with these terms."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1664-3224","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shinjyo, Noriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hikosaka, Kenji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kido, Yasutoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Norose, Kazumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-03-19"}],"displaytype":"detail","filename":"Fnimmu11_603924.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Fnimmu11_603924.pdf ","url":"https://nagasaki-u.repo.nii.ac.jp/record/26359/files/Fnimmu11_603924.pdf"},"version_id":"c493f7e1-deaa-43bb-b7b9-23279675152c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"brain","subitem_subject_scheme":"Other"},{"subitem_subject":"cerebral toxoplasmosis","subitem_subject_scheme":"Other"},{"subitem_subject":"complement","subitem_subject_scheme":"Other"},{"subitem_subject":"infection","subitem_subject_scheme":"Other"},{"subitem_subject":"microglia","subitem_subject_scheme":"Other"},{"subitem_subject":"Toxoplasma","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Toxoplasma Infection Induces Sustained Up-Regulation of Complement Factor B and C5a Receptor in the Mouse Brain via Microglial Activation: Implication for the Alternative Complement Pathway Activation and Anaphylatoxin Signaling in Cerebral Toxoplasmosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Toxoplasma Infection Induces Sustained Up-Regulation of Complement Factor B and C5a Receptor in the Mouse Brain via Microglial Activation: Implication for the Alternative Complement Pathway Activation and Anaphylatoxin Signaling in Cerebral Toxoplasmosis"}]},"item_type_id":"2","owner":"6","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-03-19"},"publish_date":"2021-03-19","publish_status":"0","recid":"26359","relation_version_is_last":true,"title":["Toxoplasma Infection Induces Sustained Up-Regulation of Complement Factor B and C5a Receptor in the Mouse Brain via Microglial Activation: Implication for the Alternative Complement Pathway Activation and Anaphylatoxin Signaling in Cerebral Toxoplasmosis"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:27:04.491084+00:00"}