{"created":"2023-05-15T16:49:23.370812+00:00","id":26824,"links":{},"metadata":{"_buckets":{"deposit":"339a5fbc-bb9f-42ac-bb13-ffc603579f21"},"_deposit":{"created_by":6,"id":"26824","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26824"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026824","sets":["35:36"]},"author_link":["118860","118866","118859","118862","118861","118864","118865","118863","118857","118858"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-04-24","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"art. no. 102358","bibliographicVolumeNumber":"83","bibliographic_titles":[{"bibliographic_title":"Parasitology International"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Plasmodium falciparum malaria parasites export several hundred proteins to the cytoplasm of infected red blood cells (RBCs) to modify the cell environment suitable for their growth. A Plasmodium translocon of exported proteins (PTEX) is necessary for both soluble and integral membrane proteins to cross the parasitophorous vacuole (PV) membrane surrounding the parasite inside the RBC. However, the molecular composition of the translocation complex for integral membrane proteins is not fully characterized, especially at the parasite plasma membrane. To examine the translocation complex, here we used mini-SURFIN4.1, consisting of a short N-terminal region, a transmembrane region, and a cytoplasmic region of an exported integral membrane protein SURFIN4.1. We found that mini-SURFIN4.1 forms a translocation intermediate complex with core PTEX components, EXP2, HSP101, and PTEX150. We also found that several proteins are exposed to the PV space, including Pf113, an uncharacterized PTEX-associated protein. We determined that Pf113 localizes in dense granules at the merozoite stage and on the parasite periphery after RBC invasion. Using an inducible translocon-clogged mini-SURFIN4.1, we found that a stable translocation intermediate complex forms at the parasite plasma membrane and contains EXP2 and a processed form of Pf113. These results suggest a potential role of Pf113 for the translocation step of mini-SURFIN4.1, providing further insights into the translocation mechanisms for parasite integral membrane proteins.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Parasitology International, 83, art. no. 102358; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.parint.2021.102358","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13835769","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miyazaki, Shinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chitama, Ben-Yeddy Abel"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kagaya, Wataru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lucky, Amuza Byaruhanga"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zhu, Xiaotong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yahata, Kazuhide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Masayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takashima, Eizo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuboi, Takafumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaneko, Osamu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-28"}],"displaytype":"detail","filename":"PI83_102358.pdf","filesize":[{"value":"3.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PI83_102358.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26824/files/PI83_102358.pdf"},"version_id":"4b8b6630-d262-46e8-b6f4-4d0c3448e208"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Malaria","subitem_subject_scheme":"Other"},{"subitem_subject":"Trafficking","subitem_subject_scheme":"Other"},{"subitem_subject":"Erythrocyte","subitem_subject_scheme":"Other"},{"subitem_subject":"Translocation","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein complex","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Plasmodium falciparum SURFIN4.1 forms an intermediate complex with PTEX components and Pf113 during export to the red blood cell","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Plasmodium falciparum SURFIN4.1 forms an intermediate complex with PTEX components and Pf113 during export to the red blood cell"}]},"item_type_id":"2","owner":"6","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-10-28"},"publish_date":"2021-10-28","publish_status":"0","recid":"26824","relation_version_is_last":true,"title":["Plasmodium falciparum SURFIN4.1 forms an intermediate complex with PTEX components and Pf113 during export to the red blood cell"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:11:06.771775+00:00"}