{"created":"2023-05-15T16:49:23.543950+00:00","id":26828,"links":{},"metadata":{"_buckets":{"deposit":"f1465f49-da7f-4e78-b21a-73d79b2b6d9b"},"_deposit":{"created_by":6,"id":"26828","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26828"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026828","sets":["35:36"]},"author_link":["118889","118891","118886","118892","118884","118885","118893","118895","118896","118897","118894","118883","118890","118888","118887"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageStart":"art. no. e0251314","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Dengue (DEN) is a neglected tropical disease, and surveillance of dengue virus (DENV) serotypes and genotypes is critical for the early detection of outbreaks. Risk factors for outbreaks include the emergence of new genotypes and serotype shifting.\nMethodology and principal findings: To understand the genomic and viral characteristics of DENV-infected patients, we conducted a cross-sectional descriptive study among pediatric patients admitted at the 550-bedded Mandalay Children Hospital during the 2018 DEN endemic season. We conducted virus isolation, serological tests, viremia level measurement, and whole-genome sequencing. Among the 202 serum samples, we detected 85 samples with DENV (46 DENV-1, 10 DENV-3, 26 DENV-4 and three multiple serotype co-infections) via reverse transcription quantitative/real-time PCR (RT-qPCR), and we obtained 49 DENV isolates (31 DENV-1, 10 DENV-3 and 8 DEN-4). We did not detect DENV-2 in this study. The viral genome levels in serum did not differ significantly among virus serotypes, infection status (primary versus secondary) and disease severity. Based on the phylogenetic analysis, we identified DENV-1 genotype-1, DENV-4 genotype-1 and DENV-3 genotype-3 and genotype-1 which was detected for the first time. Next-generation sequencing analysis revealed greater frequencies of nonsynonymous and synonymous mutations per gene in the nonstructural genes. Moreover, mutation rates were also higher among DENV-1.\nConclusion/Significance: In conclusion, there was an increasing trend of DENV-3 cases during DENV endemic season in 2018 with the first detection of the genotype 1. However, DENV-1 has remained the predominant serotype in this study area since 2013, and we identified stop codon mutations in the DENV-1 genome. This report is the first to feature a complete genome analysis of the strains of DENV-3 and DENV-4 circulating among pediatric patients in Myanmar. This study highlighted the importance of annual surveillance for a better understanding of the molecular epidemiology of DENVs.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 16(6), art. no. e0251314; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0251314","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 Ngwe Tun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ngwe Tun, Mya Myat"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kyaw, Aung Kyaw"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nabeshima, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Soe, Aung Min"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nwe, Khine Mya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Htet, Kyaw Ko Ko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aung, Thet Htoo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Htwe, Thein Thein"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aung, Thidar"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Myaing, Su Su"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mar, Tu Tu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lwin, Ei Phyu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Thu, Hlaing Myat"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Thant, Kyaw Zin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-29"}],"displaytype":"detail","filename":"PLoS16_e0251314.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS16_e0251314.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26828/files/PLoS16_e0251314.pdf"},"version_id":"15ed6c3a-a50d-4eb8-a574-d0539ac3a444"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar"}]},"item_type_id":"2","owner":"6","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-10-29"},"publish_date":"2021-10-29","publish_status":"0","recid":"26828","relation_version_is_last":true,"title":["Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:10:52.140519+00:00"}