{"created":"2023-05-15T16:49:24.067127+00:00","id":26840,"links":{},"metadata":{"_buckets":{"deposit":"08ac9cce-afe7-4103-883e-868f60d8eed0"},"_deposit":{"created_by":6,"id":"26840","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26840"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026840","sets":["10:11"]},"author_link":["118999","119006","119004","119005","119008","119009","119002","118998","119003","118996","119001","119007","118997","119000"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"art. no. 573","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"BMC Infectious Diseases"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: The recent increase in cases of azole-resistant Aspergillus fumigatus (ARAf) infections is a major clinical concern owing to its treatment limitations. Patient-derived ARAf occurs after prolonged azole treatment in patients with aspergillosis and involves various cyp51A point mutations or non-cyp51A mutations. The prognosis of patients with chronic pulmonary aspergillosis (CPA) with patient-derived ARAf infection remains unclear. In this study, we reported the case of a patient with ARAf due to HapE mutation, as well as the virulence of the isolate.\nCase presentation: A 37-year-old male was presented with productive cough and low-grade fever. The patient was diagnosed with CPA based on the chronic course, presence of a fungus ball in the upper left lobe on chest computed tomography (CT), positivity for Aspergillus-precipitating antibody and denial of other diseases. The patient underwent left upper lobe and left S6 segment resection surgery because of repeated haemoptysis during voriconazole (VRC) treatment. The patient was postoperatively treated with VRC for 6 months. Since then, the patient was followed up without antifungal treatment but relapsed 4 years later, and VRC treatment was reinitiated. Although an azole-resistant isolate was isolated after VRC treatment, the patient did not show any disease progression in either respiratory symptoms or radiological findings. The ARAf isolated from this patient showed slow growth, decreased biomass and biofilm formation in vitro, and decreased virulence in the Galleria mellonella infection model compared with its parental strain. These phenotypes could be caused by the HapE splice site mutation.\nConclusions: This is the first to report a case demonstrating the clinical manifestation of a CPA patient infected with ARAf with a HapE splice site mutation, which was consistent with the in vitro and in vivo attenuated virulence of the ARAf isolate. These results imply that not all the ARAf infections in immunocompetent patients require antifungal treatment. Further studies on the virulence of non-cyp51A mutations in ARAf are warranted.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"BMC Infectious Diseases, 21(1), art. no. 573; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s12879-021-06279-1","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s). 2021, corrected publication 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/ licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1. 0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1471-2334","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ito, Yuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takazono, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koga, Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakano, Yuichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ashizawa, Nobuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirayama, Tatsuro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tashiro, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saijo, Tomomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Kazuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imamura, Yoshifumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyazaki, Taiga"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagihara, Katsunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Izumikawa, Koichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukae, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-08"}],"displaytype":"detail","filename":"BMCID21_573.pdf","filesize":[{"value":"871.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BMCID21_573.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26840/files/BMCID21_573.pdf"},"version_id":"5d274b63-bede-43e1-8ec1-6cc58e594c34"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chronic pulmonary aspergillosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Azole-resistant Aspergillus fumigatus","subitem_subject_scheme":"Other"},{"subitem_subject":"Virulence","subitem_subject_scheme":"Other"},{"subitem_subject":"HapE","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Clinical and experimental phenotype of azole-resistant Aspergillus fumigatus with a HapE splice site mutation: a case report","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Clinical and experimental phenotype of azole-resistant Aspergillus fumigatus with a HapE splice site mutation: a case report"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-08"},"publish_date":"2021-11-08","publish_status":"0","recid":"26840","relation_version_is_last":true,"title":["Clinical and experimental phenotype of azole-resistant Aspergillus fumigatus with a HapE splice site mutation: a case report"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:09:48.971592+00:00"}