{"created":"2023-05-15T16:49:24.195251+00:00","id":26843,"links":{},"metadata":{"_buckets":{"deposit":"bf1b20a0-500f-4082-81c4-cac77eba3343"},"_deposit":{"created_by":6,"id":"26843","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26843"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026843","sets":["10:11"]},"author_link":["119037","119029","119034","119030","119032","119035","119036","119033","119031"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"13","bibliographicPageStart":"6911","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Flavobacterium johnsoniae forms a thin spreading colony on nutrient-poor agar using gliding motility. As reported in the first paper, WT cells in the colony were sparsely embedded in self-produced extracellular polymeric matrix (EPM), while sprB cells were densely packed in immature biofilm with less matrix. The colony surface is critical for antibiotic resistance and cell survival. We have now developed the Grid Stamp-Peel method whereby the colony surface is attached to a TEM grid for negative-staining microscopy. The images showed that the top of the spreading convex WT colonies was covered by EPM with few interspersed cells. Cells exposed near the colony edge made head-to-tail and/or side-to-side contact and sometimes connected via thin filaments. Nonspreading sprB and gldG and gldK colonies had a more uniform upper surface covered by different EPMs including vesicles and filaments. The EPM of sprB, gldG, and WT colonies contained filaments ~2 nm and ~5 nm in diameter; gldK colonies did not include the latter. Every cell near the edge of WT colonies had one or two dark spots, while cells inside WT colonies and cells in SprB-, GldG-, or GldK-deficient colonies did not. Together, our results suggest that the colony surface structure depends on the capability to expand biofilm.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Molecular Sciences, 22(13), art. no. 6911; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms22136911","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Keiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Naya, Masami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hatano, Yuri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kasahata, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Yoshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Mari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takebe, Katsuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Naito, Mariko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Chikara"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-09"}],"displaytype":"detail","filename":"IJMS22_6911.pdf","filesize":[{"value":"9.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJMS22_6911.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26843/files/IJMS22_6911.pdf"},"version_id":"132c2595-db8d-4db6-b065-373654dab8f3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"focal adhesion complex","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular fibers","subitem_subject_scheme":"Other"},{"subitem_subject":"vesicle","subitem_subject_scheme":"Other"},{"subitem_subject":"cell-to-cell connections","subitem_subject_scheme":"Other"},{"subitem_subject":"transmission electron microscopy","subitem_subject_scheme":"Other"},{"subitem_subject":"Grid Stamp-Peel method","subitem_subject_scheme":"Other"},{"subitem_subject":"antibiotics resistance","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Biofilm Spreading by the Adhesin-Dependent Gliding Motility of Flavobacterium johnsoniae: 2. Role of Filamentous Extracellular Network and Cell-to-Cell Connections at the Biofilm Surface","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Biofilm Spreading by the Adhesin-Dependent Gliding Motility of Flavobacterium johnsoniae: 2. Role of Filamentous Extracellular Network and Cell-to-Cell Connections at the Biofilm Surface"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-09"},"publish_date":"2021-11-09","publish_status":"0","recid":"26843","relation_version_is_last":true,"title":["Biofilm Spreading by the Adhesin-Dependent Gliding Motility of Flavobacterium johnsoniae: 2. Role of Filamentous Extracellular Network and Cell-to-Cell Connections at the Biofilm Surface"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:09:45.631344+00:00"}