{"created":"2023-05-15T16:49:24.961347+00:00","id":26863,"links":{},"metadata":{"_buckets":{"deposit":"7548a6bc-6548-4234-a5fa-93979ca17755"},"_deposit":{"created_by":6,"id":"26863","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26863"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026863","sets":["10:11"]},"author_link":["119234","119237","119232","119240","119235","119236","119238","119233","119239"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-07-22","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"422","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"Stem Cell Research & Therapy"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Radiation-induced heart disease has been reported, but the underlying mechanisms remain unclear. Mesenchymal stem cells (MSCs), also residing in the heart, are highly susceptible to radiation. We examined the hypothesis that the altered secretion of extracellular vesicles (EVs) from MSCs is the trigger of radiation-induced heart disease.\nMethods: By exposing human placental tissue-derived MSCs to 5 Gy γ-rays, we then isolated EVs from the culture medium 48 h later and evaluated the changes in quantity and quality of EVs from MSCs after radiation exposure. The biological effects of EVs from irradiated MSCs on HUVECs and H9c2 cells were also examined.\nResults: Although the amount and size distribution of EVs did not differ between the nonirradiated and irradiated MSCs, miRNA sequences indicated many upregulated or downregulated miRNAs in irradiated MSCs EVs. In vitro experiments using HUVEC and H9c2 cells showed that irradiated MSC-EVs decreased cell proliferation (P < 0.01), but increased cell apoptosis and DNA damage. Moreover, irradiated MSC-EVs impaired the HUVEC tube formation and induced calcium overload in H9c2 cells.\nConclusions: EVs released from irradiated MSCs show altered miRNA profiles and harmful effects on heart cells, which provides new insight into the mechanism of radiation-related heart disease risks.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Stem Cell Research and Therapy, 12, art. no. 422; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s13287-021-02504-5","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s). 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1757-6512","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Luo, Lan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yan, Chen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fuchi, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kodama, Yukinobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zhang, Xu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shinji, Goto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miura, Kiyonori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Li, Tao-Sheng"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-16"}],"displaytype":"detail","filename":"SCRT12_422.pdf","filesize":[{"value":"3.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SCRT12_422.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26863/files/SCRT12_422.pdf"},"version_id":"1d0c65aa-40b3-49e4-9ce0-8e034de3e163"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mesenchymal stem cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Extracellular vesicles","subitem_subject_scheme":"Other"},{"subitem_subject":"Ionizing radiation","subitem_subject_scheme":"Other"},{"subitem_subject":"Heart disease","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mesenchymal stem cell-derived extracellular vesicles as probable triggers of radiation-induced heart disease","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mesenchymal stem cell-derived extracellular vesicles as probable triggers of radiation-induced heart disease"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-16"},"publish_date":"2021-11-16","publish_status":"0","recid":"26863","relation_version_is_last":true,"title":["Mesenchymal stem cell-derived extracellular vesicles as probable triggers of radiation-induced heart disease"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:08:45.703295+00:00"}