{"created":"2023-05-15T16:49:25.223891+00:00","id":26869,"links":{},"metadata":{"_buckets":{"deposit":"2b43d8fb-81e2-4e4b-ba91-c982434f22f8"},"_deposit":{"created_by":6,"id":"26869","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26869"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026869","sets":["25:26"]},"author_link":["119295","119294","119292","119297","119291","119296","119293","119298"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-07-13","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageStart":"1073","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"Pharmaceutics"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The accumulation of high levels of 99mTc-tetrofosmin (99mTc-TF) in the hepatobiliary system can lead to imaging artifacts and interference with diagnosis. The present study investigated the transport mechanisms of 99mTc-TF and attempted to apply competitive inhibition using a specific inhibitor to reduce 99mTc-TF hepatic accumulation. In this in vitro study, 99mTc-TF was incubated in HEK293 cells expressing human organic anion transporting polypeptide 1B1 (OATP1B1), OATP1B3, OATP2B1, organic anion transporter 2 (OAT2), organic cation transporter 1 (OCT1), OCT2, and Na+-taurocholate cotransporting polypeptide with or without each specific inhibitor to evaluate the contribution of each transporter to 99mTc-TF transportation. In vivo studies, dynamic planar imaging, and single photon emission computed tomography (SPECT) experiments with rats were performed to observe alterations to 99mTc-TF pharmacokinetics using cimetidine (CMT) as an OCT1 inhibitor. Time–activity curves in the liver and heart were acquired from dynamic data, and the 99mTc-TF uptake ratio was calculated from SPECT. From the in vitro study, 99mTc-TF was found to be transported by OCT1 and OCT2. When CMT-preloaded rats and control rats were compared, the hepatic accumulation of the 99mTc-TF was reduced, and the time to peak heart count shifted to an earlier stage. The hepatic accumulation of 99mTc-TF was markedly suppressed, and the heart-to-liver ratio increased 1.6-fold. The pharmacokinetics of 99mTc-TF were greatly changed by OCT1 inhibitor. Even in humans, the administration of OCT1 inhibitor before cardiac SPECT examination may reduce 99mTc-TF hepatic accumulation and contribute to the suppression of artifacts and the improvement of SPECT image quality. ","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Pharmaceutics, 13(7), art. no. 1073; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/pharmaceutics13071073","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1999-4923","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nishi, Kodai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kikuchi, Minori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mizutani, Asuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muranaka, Yuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tamai, Ikumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawai, Keiichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kudo, Takashi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-18"}],"displaytype":"detail","filename":"Pharmaceutics13_1073.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Pharmaceutics13_1073.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26869/files/Pharmaceutics13_1073.pdf"},"version_id":"f3125dd5-59ef-4808-a85f-ff518a59dd7a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"99mTc-tetrofosmin","subitem_subject_scheme":"Other"},{"subitem_subject":"small-animal SPECT/CT","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatic uptake","subitem_subject_scheme":"Other"},{"subitem_subject":"transport mechanism","subitem_subject_scheme":"Other"},{"subitem_subject":"OCT1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Inhibition of the Hepatic Uptake of 99mTc-Tetrofosmin Using an Organic Cation Transporter Blocker","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inhibition of the Hepatic Uptake of 99mTc-Tetrofosmin Using an Organic Cation Transporter Blocker"}]},"item_type_id":"2","owner":"6","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-18"},"publish_date":"2021-11-18","publish_status":"0","recid":"26869","relation_version_is_last":true,"title":["Inhibition of the Hepatic Uptake of 99mTc-Tetrofosmin Using an Organic Cation Transporter Blocker"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:08:27.296018+00:00"}