@article{oai:nagasaki-u.repo.nii.ac.jp:00026879,
 author = {Shibata, Takayuki and Shimamura, Ryosuke and Yamamoto, Yuji and Sakurai, Hiroki and Fujita, Junya and Yamayoshi, Asako and Nemoto, Toshimitsu and Kabashima, Tsutomu},
 issue = {8},
 journal = {Chemical and Pharmaceutical Bulletin},
 month = {Aug},
 note = {A  facile  and  reliable  fluorescence  method  for  the  quantification  of  urinary  uracil  concentration  is  pro-posed herein. The assay utilizes a specific fluorescence (FL) derivatization reaction for uracil using 3-methyl-benzamidoxime  as  a  fluorogenic  reagent.  Although  the  presence  of  urine  inhibited  the  FL  reaction,  10 μL  of urine was sufficient for the detection of urinary uracil. The uracil derivative was successfully separated from other  fluorescent  impurities  using  simple  reversed-phase  LC  with  FL  detection.  Urinary  uracil  concentra-tions  from  16  people  were  compared  with  the  concentrations  obtained  by  the  traditional  column-switching liquid chromatographic analysis with UV detection. The FL derivative of uracil appeared as a single peak in the chromatograms of all samples. However, several samples showed an additional peak overlapping the ura-cil  peak  when  using  the  column-switching  method  because  of  UV-active  impurities.  These  results  indicated that that the present method is not affected by interfering substances in urine and affords a precise determi-nation of urinary uracil. We expect the proposed method to be applicable for diagnosing dihydropyrimidine dehydrogenase deficiency in 5-fluorouracil chemotherapy., Chemical and Pharmaceutical Bulletin, 69(8), pp. 768-772; 2021},
 pages = {768--772},
 title = {A Facile Method for the Quantification of Urinary Uracil Concentration by a Uracil-Specific Fluorescence Derivatization Reaction},
 volume = {69},
 year = {2021}
}