{"created":"2023-05-15T16:49:26.250111+00:00","id":26894,"links":{},"metadata":{"_buckets":{"deposit":"558faa8d-2b91-4526-96ef-5e9fb1676c57"},"_deposit":{"created_by":6,"id":"26894","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26894"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026894","sets":["10:11"]},"author_link":["119530","119529","119520","119519","119524","119527","119518","119526","119525","119521","119528","119531","119523","119522"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-08-16","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageStart":"1264","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"Pharmaceutics"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"With the emergence of coronavirus disease-2019, researchers have gained interest in the therapeutic efficacy of mesenchymal stem/stromal cells (MSCs) in acute respiratory distress syndrome; however, the mechanisms of the therapeutic effects of MSCs are unclear. We have previously reported that adipose-derived MSCs (AD-MSCs) strengthen the barrier function of the pulmonary vessels in scaffold-based bioengineered rat lungs. In this study, we evaluated whether AD-MSCs could enhance the intercellular barrier function of lung epithelial cells in vitro using a transwell coculture system. Transepithelial electrical resistance (TEER) measurements revealed that the peak TEER value was significantly higher in the AD-MSC coculture group than in the AD-MSC non-coculture group. Similarly, the permeability coefficient was significantly decreased in the AD-MSC coculture group compared to that in the AD-MSC non-coculture group. Immunostaining of insert membranes showed that zonula occuldens-1 expression was significantly high at cell junctions in the AD-MSC coculture group. Moreover, cell junction-related gene profiling showed that the expression of some claudin genes, including claudin-4, was upregulated in the AD-MSC coculture group. Taken together, these results showed that AD-MSCs enhanced the barrier function between lung epithelial cells, suggesting that both direct adhesion and indirect paracrine effects strengthened the barrier function of lung alveolar epithelium in vitro.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Pharmaceutics, 13(8), art. no. 1264; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/pharmaceutics13081264","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1999-4923","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishii, Mitsutoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuchiya, Tomoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Doi, Ryoichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morofuji, Yoichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujimoto, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muto, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suematsu, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mori, Ryoichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumoto, Keitaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyazaki, Takuro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomoshige, Koichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Hironosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwatake, Mayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagayasu, Takeshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-25"}],"displaytype":"detail","filename":"Pharmaceutics13_1264.pdf","filesize":[{"value":"6.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Pharmaceutics13_1264.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26894/files/Pharmaceutics13_1264.pdf"},"version_id":"94149e5c-6cd6-4f5e-8197-7a9b5ffdc59c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"mesenchymal stem/stromal cell","subitem_subject_scheme":"Other"},{"subitem_subject":"alveolar epithelial cell","subitem_subject_scheme":"Other"},{"subitem_subject":"barrier function","subitem_subject_scheme":"Other"},{"subitem_subject":"air-blood barrier","subitem_subject_scheme":"Other"},{"subitem_subject":"transepithelial electrical resistance","subitem_subject_scheme":"Other"},{"subitem_subject":"acute respiratory distress syndrome","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-25"},"publish_date":"2021-11-25","publish_status":"0","recid":"26894","relation_version_is_last":true,"title":["Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:07:26.501081+00:00"}