{"created":"2023-05-15T16:49:26.895630+00:00","id":26908,"links":{},"metadata":{"_buckets":{"deposit":"30155958-72d1-4969-affc-6051d278fe24"},"_deposit":{"created_by":6,"id":"26908","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26908"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026908","sets":["10:11"]},"author_link":["119668","119665","119672","119661","119662","119666","119664","119667","119671","119670","119669","119663"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-09-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"18","bibliographicPageStart":"9845","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"International Journal of Environmental Research and Public Health"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Infectivity and neutralizing antibody titers of flavivirus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are frequently measured using the conventional plaque assay. While the assay is useful in the determination of infectivity, conventional plaque assays generally possess lower sensitivity and are time-consuming compared to nucleic acid amplification tests. In this study, a microcrystalline cellulose (MCC), Avicel, was evaluated as an alternative to the conventional virus overlay medium, methylcellulose, for a plaque assay. The plaque assay was performed using dengue and COVID-19 clinical samples and laboratory-established flavivirus and SARS-CoV-2 strains. In virus titration of clinical samples, the plaques were significantly larger, and the virus titers were higher when Avicel MCC-containing overlay medium was used than with conventional methylcellulose overlay medium. In addition, for some clinical samples and laboratory virus strains, infectious particles were detected as plaques in the Avicel MCC-containing medium, but not in the conventional methylcellulose medium. The results suggest that the viremia titer determined using the new overlay medium containing Avicel MCC may better reflect the innate infectious and plaque-forming capabilities of clinical samples and better reflect virus infectivity.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Environmental Research and Public Health, 18(18), art. no. 9845; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijerph18189845","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1660-4601","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fukuta, Mizuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nguyen, Co Thach"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nguyen, Thi Thu Thuy"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nguyen, Thi Thanh Ngan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Vu, Thi Bich Hau"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takemura, Taichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nguyen, Le Khanh Hang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Shingo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Le, Thi Quynh Mai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hasebe, Futoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Moi, Meng Ling"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-12-02"}],"displaytype":"detail","filename":"IJERPH18_9845.pdf","filesize":[{"value":"956.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJERPH18_9845.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26908/files/IJERPH18_9845.pdf"},"version_id":"6151f631-00cc-4394-b3c6-cfd8be68000f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"infectivity assessment","subitem_subject_scheme":"Other"},{"subitem_subject":"infectious particles","subitem_subject_scheme":"Other"},{"subitem_subject":"dengue","subitem_subject_scheme":"Other"},{"subitem_subject":"SARS-CoV-2","subitem_subject_scheme":"Other"},{"subitem_subject":"culture medium","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Discrepancies in Infectivity of Flavivirus and SARS-CoV-2 Clinical Samples: An Improved Assay for Infectious Virus Shedding and Viremia Assessment","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Discrepancies in Infectivity of Flavivirus and SARS-CoV-2 Clinical Samples: An Improved Assay for Infectious Virus Shedding and Viremia Assessment"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-02"},"publish_date":"2021-12-02","publish_status":"0","recid":"26908","relation_version_is_last":true,"title":["Discrepancies in Infectivity of Flavivirus and SARS-CoV-2 Clinical Samples: An Improved Assay for Infectious Virus Shedding and Viremia Assessment"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:06:54.376052+00:00"}