{"created":"2023-05-15T16:49:26.939554+00:00","id":26909,"links":{},"metadata":{"_buckets":{"deposit":"a56179ec-3823-47b9-b0ac-ea602df9434a"},"_deposit":{"created_by":6,"id":"26909","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26909"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026909","sets":["35:36"]},"author_link":["119675","119674","119686","119690","119683","119676","119681","119685","119689","119684","119679","119680","119687","119673","119677","119682","119688","119678"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-09-13","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"18","bibliographicPageStart":"9630","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"International Journal of Environmental Research and Public Health"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). Real-time RT-PCR is the most commonly used method for COVID-19 diagnosis. However, serological assays are urgently needed as complementary tools to RT-PCR. Hachim et al. 2020 and Burbelo et al. 2020 demonstrated that anti-nucleocapsid(N) SARS-CoV-2 antibodies are higher and appear earlier than the spike antibodies. Additionally, cross-reactive antibodies against N protein are more prevalent than those against spike protein. We developed a less cross-reactive immunoglobulin G (IgG) indirect ELISA by using a truncated recombinant SARS-CoV-2 N protein as assay antigen. A highly conserved region of coronaviruses N protein was deleted and the protein was prepared using an E. coli protein expression system. A total of 177 samples collected from COVID-19 suspected cases and 155 negative control sera collected during the pre-COVID-19 period were applied to evaluate the assay’s performance, with the plaque reduction neutralization test and the commercial SARS-CoV-2 spike protein IgG ELISA as gold standards. The SARS-CoV-2 N truncated protein-based ELISA showed similar sensitivity (91.1% vs. 91.9%) and specificity (93.8% vs. 93.8%) between the PRNT and spike IgG ELISA, as well as also higher specificity compared to the full-length N protein (93.8% vs. 89.9%). Our ELISA can be used for the diagnosis and surveillance of COVID-19.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Environmental Research and Public Health, 18(18), art. no. 9630; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijerph18189630","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1660-4601","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mutantu, Pierre Nsele"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ngwe Tun, Mya Myat"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nabeshima, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yu, Fuxun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukadi, Patrick Kakoni"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tashiro, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujita, Ayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanie, Nobuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oshiro, Ryosaku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takazono, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imamura, Yoshifumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirayama, Tatsuro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Moi, Meng Ling"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Shingo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Izumikawa, Koichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuda, Jiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-12-02"}],"displaytype":"detail","filename":"IJERPH18_9630.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJERPH18_9630.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26909/files/IJERPH18_9630.pdf"},"version_id":"660d289f-fb08-4577-b4e4-481526a78806"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SARS-CoV-2","subitem_subject_scheme":"Other"},{"subitem_subject":"nucleocapsid protein","subitem_subject_scheme":"Other"},{"subitem_subject":"IgG indirect ELISA","subitem_subject_scheme":"Other"},{"subitem_subject":"plaque reduction neutralization test","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen"}]},"item_type_id":"2","owner":"6","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-02"},"publish_date":"2021-12-02","publish_status":"0","recid":"26909","relation_version_is_last":true,"title":["Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:06:46.492899+00:00"}