@article{oai:nagasaki-u.repo.nii.ac.jp:00026928, author = {Maekawa, Kyoichiro and Natsuda, Koji and Hidaka, Masaaki and Uematsu, Masafumi and Soyama, Akihiko and Hara, Takanobu and Takatsuki, Mitsuhisa and Nagai, Kazuhiro and Miura, Kiyonori and Eguchi, Susumu}, journal = {Regenerative Therapy}, month = {Sep}, note = {Amniotic membrane is attracting attention as a new material for regenerative medicine. We herein report that the culture of primary rat hepatocytes on human amniotic membrane maintained their morphology and their production of albumin for at least two months. Human amniotic membrane was collected during planned cesarean section and kept frozen until usage. Primary rat hepatocytes were plated on human amniotic membrane. Hepatocytes accumulated as colonies on amniotic membrane, and their rat albumin level was maintained for two months. Their three-dimensional structure on extracellular matrix, which is abundant in amniotic membranes might influence the maintenance of the hepatocyte-specific function., Regenerative Therapy, 18, pp. 384-390; 2021}, pages = {384--390}, title = {Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate}, volume = {18}, year = {2021} }