{"created":"2023-05-15T16:49:28.923889+00:00","id":26955,"links":{},"metadata":{"_buckets":{"deposit":"0d853c82-bb96-42a3-997d-2b4f41deaf05"},"_deposit":{"created_by":6,"id":"26955","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"26955"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00026955","sets":["8:9"]},"author_link":["120005","120014","120013","120011","120007","120006","120004","120016","120012","120009","120010","120008","120015"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-10-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"21","bibliographicPageStart":"11561","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We aimed to investigate the effect of methotrexate (MTX) on microRNA modulation in rheumatoid arthritis fibroblast-like synovial cells (RA-FLS). RA-FLS were treated with MTX for 48 h. We then performed miRNA array analysis to investigate differentially expressed miRNAs. Transfection with miR-877-3p precursor and inhibitor were used to investigate the functional role of miR-877-3p in RA-FLS. Gene ontology analysis was used to investigate the cellular processes involving miR-877-3p. The production of cytokines/chemokines was screened by multiplex cytokine/chemokine bead assay and confirmed by ELISA and quantitative real-time PCR. The migratory and proliferative activities of RA-FLS were analyzed by wound healing assay and MKI-67 expression. MTX treatment altered the expression of 13 miRNAs (seven were upregulated and six were downregulated). Among them, quantitative real-time PCR confirmed that miR-877-3p was upregulated in response to MTX (1.79 ± 0.46-fold, p < 0.05). The possible target genes of miR-877-3p in RA-FLS revealed by the microarray analysis were correlated with biological processes. The overexpression of miR-877-3p decreased the production of GM-CSF and CCL3, and the overexpression of miR-877-3p inhibited migratory and proliferative activity. MTX altered the miR-877-3p expression on RA-FLS, and this alteration of miR-877-3p attenuated the abundant production of cytokines/chemokines and proliferative property of RA-FLS.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Molecular Sciences, 22(21), art. no. 11561; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms222111561","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Iwamoto, Naoki"}],"nameIdentifiers":[{"nameIdentifier":"120004","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"120005","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Endo, Yushiro"}],"nameIdentifiers":[{"nameIdentifier":"120006","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Toshimasa"}],"nameIdentifiers":[{"nameIdentifier":"120007","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sumiyoshi, Remi"}],"nameIdentifiers":[{"nameIdentifier":"120008","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Umeda, Masataka"}],"nameIdentifiers":[{"nameIdentifier":"120009","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koga, Tomohiro"}],"nameIdentifiers":[{"nameIdentifier":"120010","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawashiri, Shin-ya"}],"nameIdentifiers":[{"nameIdentifier":"120011","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Igawa, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"120012","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichinose, Kunihiro"}],"nameIdentifiers":[{"nameIdentifier":"120013","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tamai, Mami"}],"nameIdentifiers":[{"nameIdentifier":"120014","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Origuchi, Tomoki"}],"nameIdentifiers":[{"nameIdentifier":"120015","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawakami, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"120016","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-12-22"}],"displaytype":"detail","filename":"IJMS22_11561.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJMS22_11561.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/26955/files/IJMS22_11561.pdf"},"version_id":"c671b3df-b707-420c-a818-aa43445a00ce"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MTX","subitem_subject_scheme":"Other"},{"subitem_subject":"miR-877-3p","subitem_subject_scheme":"Other"},{"subitem_subject":"rheumatoid arthritis","subitem_subject_scheme":"Other"},{"subitem_subject":"fibroblast-like synovial cells","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Methotrexate Alters the Expression of microRNA in Fibroblast-like Synovial Cells in Rheumatoid Arthritis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Methotrexate Alters the Expression of microRNA in Fibroblast-like Synovial Cells in Rheumatoid Arthritis"}]},"item_type_id":"2","owner":"6","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-22"},"publish_date":"2021-12-22","publish_status":"0","recid":"26955","relation_version_is_last":true,"title":["Methotrexate Alters the Expression of microRNA in Fibroblast-like Synovial Cells in Rheumatoid Arthritis"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:04:52.181215+00:00"}