{"created":"2023-05-15T16:49:31.566199+00:00","id":27017,"links":{},"metadata":{"_buckets":{"deposit":"5931f9f2-d9ea-45f7-a6b7-3dee19b2c08f"},"_deposit":{"created_by":6,"id":"27017","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27017"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027017","sets":["10:11"]},"author_link":["120251","120253","120254","120246","120250","120252","120248","120249","120247","120245"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-11-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicPageStart":"12434","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Dental calculus (DC) is a common deposit in periodontitis patients. We have previously shown that DC contains both microbial components and calcium phosphate crystals that induce an osteoclastogenic cytokine IL-1β via the NLRP3 inflammasome in macrophages. In this study, we examined the effects of cytokines produced by mouse macrophages stimulated with DC on osteoclastogenesis. The culture supernatants from wild-type (WT) mouse macrophages stimulated with DC accelerated osteoclastogenesis in RANKL-primed mouse bone marrow macrophages (BMMs), but inhibited osteoclastogenesis in RANKL-primed RAW-D cells. WT, but not NLRP3-deficient, mouse macrophages stimulated with DC produced IL-1β and IL-18 in a dose-dependent manner, indicating the NLRP3 inflammasome-dependent production of IL-1β and IL-18. Both WT and NLRP3-deficient mouse macrophages stimulated with DC produced IL-10, indicating the NLRP3 inflammasome-independent production of IL-10. Recombinant IL-1β accelerated osteoclastogenesis in both RANKL-primed BMMs and RAW-D cells, whereas recombinant IL-18 and IL-10 inhibited osteoclastogenesis. These results indicate that DC induces osteoclastogenic IL-1β in an NLRP3 inflammasome-dependent manner and anti-osteogenic IL-18 and IL-10 dependently and independently of the NLRP3 inflammasome, respectively. DC may promote alveolar bone resorption via IL-1β induction in periodontitis patients, but suppress resorption via IL-18 and IL-10 induction in some circumstances.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Molecular Sciences, 22(22), art. no. 12434; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms222212434","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mae, Megumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Alam, Mohammad Ibtehaz"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Yasunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ozaki, Yukio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Higuchi, Kanako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ziauddin, S. M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Montenegro Raudales, Jorge Luis"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakai, Eiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsukuba, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshimura, Atsutoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-24"}],"displaytype":"detail","filename":"IJMS22_12434.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJMS22_12434.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27017/files/IJMS22_12434.pdf"},"version_id":"5c0ef411-493d-42f8-a19c-a50e7e4b94a4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"dental calculus","subitem_subject_scheme":"Other"},{"subitem_subject":"NLRP3 inflammasome","subitem_subject_scheme":"Other"},{"subitem_subject":"IL-1β","subitem_subject_scheme":"Other"},{"subitem_subject":"IL-18","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoclast","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Role of Cytokines Produced via the NLRP3 Inflammasome in Mouse Macrophages Stimulated with Dental Calculus in Osteoclastogenesis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Role of Cytokines Produced via the NLRP3 Inflammasome in Mouse Macrophages Stimulated with Dental Calculus in Osteoclastogenesis"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-24"},"publish_date":"2022-01-24","publish_status":"0","recid":"27017","relation_version_is_last":true,"title":["The Role of Cytokines Produced via the NLRP3 Inflammasome in Mouse Macrophages Stimulated with Dental Calculus in Osteoclastogenesis"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:03:09.751671+00:00"}