{"created":"2023-05-15T16:49:32.162466+00:00","id":27031,"links":{},"metadata":{"_buckets":{"deposit":"fbae6d45-9736-48cd-a339-6bb7fbdfcd66"},"_deposit":{"created_by":6,"id":"27031","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27031"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027031","sets":["8:9"]},"author_link":["120360","120366","120362","120361","120364","120363","120367","120365"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-11-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageStart":"1983","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"Pharmaceutics"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In a previous study, we constructed a lung-targeting lipopolyplex containing polyethyleneimine (PEI), 1,2-di-O-octadecenyl-3-trimethylammonium propane (DOTMA), and N-lauroylsarcosine (LS). The lipopolyplex exhibited an extremely high gene expression in the lung after intravenous administration. Here, we optimized the lipopolyplex and used it to deliver a TGF-β1 shRNA to treat refractory pulmonary fibrosis. We constructed several lipopolyplexes with pDNA, various cationic polymers, cationic lipids, and LS to select the most effective formulation. Then, the pDNA encoding shRNA against mouse TGF-β1 was encapsulated in the lipopolyplex and injected into mice with bleomycin-induced pulmonary fibrosis. After optimizing the lipopolyplex, dendrigraft poly-L-lysine (DGL) and DOTMA were selected as the appropriate cationic polymer and lipid, respectively. The lipopolyplex was constructed with a pDNA, DGL, DOTMA, and LS charge ratio of 1:2:2:4 showed the highest gene expression. After intravenous administration of the lipopolyplex, the highest gene expression was observed in the lung. In the in vitro experiment, the lipopolyplex delivered pDNA into the cells via endocytosis. As a result, the lipopolyplex containing pDNA encoding TGF-β1 shRNA significantly decreased hydroxyproline in the pulmonary fibrosis model mice. We have successfully inhibited pulmonary fibrosis using a novel lung-targeting lipopolyplex.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Pharmaceutics, 13(11), art. no. 1983; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/pharmaceutics13111983","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1999-4923","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kurosaki, Tomoaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanda, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashizume, Junya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Kayoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Harasawa, Hitomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tadahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kodama, Yukinobu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-28"}],"displaytype":"detail","filename":"Pharmaceutics13_1983.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Pharmaceutics13_1983.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27031/files/Pharmaceutics13_1983.pdf"},"version_id":"02e467ae-6065-425f-a9bb-369d2a17a4cc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"gene delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"shRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"nanoparticles","subitem_subject_scheme":"Other"},{"subitem_subject":"pulmonary fibrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"N-lauroylsarcosine","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Delivery of pDNA to the Lung by Lipopolyplexes Using N-Lauroylsarcosine and Effect on the Pulmonary Fibrosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Delivery of pDNA to the Lung by Lipopolyplexes Using N-Lauroylsarcosine and Effect on the Pulmonary Fibrosis"}]},"item_type_id":"2","owner":"6","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-28"},"publish_date":"2022-01-28","publish_status":"0","recid":"27031","relation_version_is_last":true,"title":["Delivery of pDNA to the Lung by Lipopolyplexes Using N-Lauroylsarcosine and Effect on the Pulmonary Fibrosis"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:02:30.794630+00:00"}