@article{oai:nagasaki-u.repo.nii.ac.jp:00027055,
 author = {Mao, Zhan Qiu and Fukuta, Mizuki and Balingit, Jean Claude and Nguyen, Thi Thanh Ngan and Nguyen, Co Thach and Inoue, Shingo and Nguyen, Thi Thu Thuy and Nguyen, Le Khanh Hang and Minakawa, Noboru and Morita, Kouichi and Le, Thi Quynh Mai and Hasebe, Futoshi and Moi, Meng Ling},
 issue = {12},
 journal = {Pathogens},
 month = {Nov},
 note = {The RT-qPCR method remains the gold standard and first-line diagnostic method for the detection of SARS-CoV-2 and flaviviruses, especially in the early stage of viral infection. Rapid and accurate viral detection is a starting point in the containment of the COVID-19 pandemic and flavivirus outbreaks. However, the shortage of diagnostic reagents and supplies, especially in resource-limited countries that experience co-circulation of SARS-CoV-2 and flaviviruses, are limitations that may result in lesser availability of RT-qPCR-based diagnostic tests. In this study, the utility of RNA-free extraction methods was assessed for the direct detection of SARS-CoV-2 and DENV-2 in heat-inactivated or chemical-inactivated samples. The findings demonstrate that direct real-time RT-qPCR is a feasible option in comparison to conventional real-time RT-qPCR based on viral genome extraction-based methods. The utility of heat-inactivation and direct real-time RT-qPCR for SARS-CoV-2, DENV-2 viral RNA detection was demonstrated by using clinical samples of SARS-CoV-2 and DENV-2 and spiked cell culture samples of SARS-CoV-2 and DENV-2. This study provides a simple alternative workflow for flavivirus and SARS-CoV-2 detection that includes heat inactivation and viral RNA extraction-free protocols, with aims to reduce the risk of exposure during processing of SARS-CoV-2 biological specimens and to overcome the supply-chain bottleneck, particularly in resource limited settings with flavivirus co-circulation., Pathogens, 10(12), art. no. 1558; 2021},
 title = {Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated Samples by Real-Time RT-qPCR: Implications for Diagnosis in Resource Limited Settings with Flavivirus Co-Circulation},
 volume = {10},
 year = {2021}
}