{"created":"2023-05-15T16:49:33.197881+00:00","id":27055,"links":{},"metadata":{"_buckets":{"deposit":"207399f5-b5e8-487e-9361-693ff378c72c"},"_deposit":{"created_by":6,"id":"27055","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27055"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027055","sets":["35:36"]},"author_link":["120475","120478","120470","120466","120476","120471","120474","120468","120467","120473","120477","120469","120472"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-11-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageStart":"1558","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Pathogens"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The RT-qPCR method remains the gold standard and first-line diagnostic method for the detection of SARS-CoV-2 and flaviviruses, especially in the early stage of viral infection. Rapid and accurate viral detection is a starting point in the containment of the COVID-19 pandemic and flavivirus outbreaks. However, the shortage of diagnostic reagents and supplies, especially in resource-limited countries that experience co-circulation of SARS-CoV-2 and flaviviruses, are limitations that may result in lesser availability of RT-qPCR-based diagnostic tests. In this study, the utility of RNA-free extraction methods was assessed for the direct detection of SARS-CoV-2 and DENV-2 in heat-inactivated or chemical-inactivated samples. The findings demonstrate that direct real-time RT-qPCR is a feasible option in comparison to conventional real-time RT-qPCR based on viral genome extraction-based methods. The utility of heat-inactivation and direct real-time RT-qPCR for SARS-CoV-2, DENV-2 viral RNA detection was demonstrated by using clinical samples of SARS-CoV-2 and DENV-2 and spiked cell culture samples of SARS-CoV-2 and DENV-2. This study provides a simple alternative workflow for flavivirus and SARS-CoV-2 detection that includes heat inactivation and viral RNA extraction-free protocols, with aims to reduce the risk of exposure during processing of SARS-CoV-2 biological specimens and to overcome the supply-chain bottleneck, particularly in resource limited settings with flavivirus co-circulation.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Pathogens, 10(12), art. no. 1558; 2021","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/pathogens10121558","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"2076-0817","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mao, Zhan Qiu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuta, Mizuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Balingit, Jean Claude"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nguyen, Thi Thanh Ngan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nguyen, Co Thach"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Shingo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nguyen, Thi Thu Thuy"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nguyen, Le Khanh Hang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Minakawa, Noboru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Le, Thi Quynh Mai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hasebe, Futoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Moi, Meng Ling"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-02-07"}],"displaytype":"detail","filename":"Pathogens10_1558.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Pathogens10_1558.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27055/files/Pathogens10_1558.pdf"},"version_id":"338af77f-7848-4e2a-aeab-c4a847e1c692"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SARS-CoV-2","subitem_subject_scheme":"Other"},{"subitem_subject":"DENV","subitem_subject_scheme":"Other"},{"subitem_subject":"virus co-circulation","subitem_subject_scheme":"Other"},{"subitem_subject":"direct RT-qPCR","subitem_subject_scheme":"Other"},{"subitem_subject":"virus inactivation","subitem_subject_scheme":"Other"},{"subitem_subject":"biosafety","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated Samples by Real-Time RT-qPCR: Implications for Diagnosis in Resource Limited Settings with Flavivirus Co-Circulation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated Samples by Real-Time RT-qPCR: Implications for Diagnosis in Resource Limited Settings with Flavivirus Co-Circulation"}]},"item_type_id":"2","owner":"6","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-02-07"},"publish_date":"2022-02-07","publish_status":"0","recid":"27055","relation_version_is_last":true,"title":["Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated Samples by Real-Time RT-qPCR: Implications for Diagnosis in Resource Limited Settings with Flavivirus Co-Circulation"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T20:01:29.883344+00:00"}