{"created":"2023-05-15T16:49:45.165719+00:00","id":27336,"links":{},"metadata":{"_buckets":{"deposit":"0df6b1ca-7696-4785-89dd-a54dd3732022"},"_deposit":{"created_by":6,"id":"27336","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27336"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027336","sets":["35:36"]},"author_link":["121618","121617","121623","121621","121622","121619","121624","121620"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageStart":"e0010274","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"PLoS Neglected Tropical Diseases"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Mycetoma is a tropical disease caused by several fungi and bacteria present in the soil. Fungal mycetoma and eumycetoma are especially challenging to treat; therefore, prevention, early diagnosis, and early treatment are important, but it is also necessary to understand the geographic distribution of these pathogenic fungi. In this study, we used DNA metabarcoding methodology to identify fungal species from soil samples. Soil sampling was implemented at seven villages in an endemic area of Sennar State in Sudan in 2019, and ten sampling sites were selected in each village according to land-use conditions. In total, 70 soil samples were collected from ground surfaces, and DNA in the soil was extracted with a combined method of alkaline DNA extraction and a commercial soil DNA extraction kit. The region for universal primers was selected to be the ribosomal internal transcribed spacer one region for metabarcoding. After the second PCR for DNA library preparation, the amplicon-based DNA analysis was performed using next-generation sequencing with two sets of universal primers. A total of twelve mycetoma-causative fungal species were identified, including the prime agent, Madurella mycetomatis, and additional pathogens, Falciformispora senegalensis and Falciformispora tompkinsii, in 53 soil samples. This study demonstrated that soil DNA metabarcoding can elucidate the presence of multiple mycetomacausative fungi, which may contribute to accurate diagnosis for patient treatment and geographical mapping.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLOS Neglected Tropical Diseases, 16(3), art. no. e0010274; 2022","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pntd.0010274","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 Hashizume et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"19352727","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hashizume, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taga, Suguru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakata, Masayuki K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taha, Mahmoud Hussein Mohamed"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Siddig, Emmanuel Edwar"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Minamoto, Toshifumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fahal, Ahmed Hassan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaneko, Satoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-26"}],"displaytype":"detail","filename":"PNTD16_e0010274.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PNTD16_e0010274.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27336/files/PNTD16_e0010274.pdf"},"version_id":"f3195d0d-a734-4e80-adfd-a6d2e05338e1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Detection of multiple mycetoma pathogens using fungal metabarcoding analysis of soil DNA in an endemic area of Sudan","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of multiple mycetoma pathogens using fungal metabarcoding analysis of soil DNA in an endemic area of Sudan"}]},"item_type_id":"2","owner":"6","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-04-26"},"publish_date":"2022-04-26","publish_status":"0","recid":"27336","relation_version_is_last":true,"title":["Detection of multiple mycetoma pathogens using fungal metabarcoding analysis of soil DNA in an endemic area of Sudan"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T19:51:35.910423+00:00"}