{"created":"2023-05-15T16:49:45.989408+00:00","id":27357,"links":{},"metadata":{"_buckets":{"deposit":"fca06d3f-dfc9-4e1c-8e4f-dfbe776c3bef"},"_deposit":{"created_by":6,"id":"27357","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27357"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027357","sets":["35:36"]},"author_link":["121733","121736","121727","121728","121735","121730","121732","121731","121729","121734"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-04-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageStart":"816","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Viruses"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lamellarin α20-sulfate is a cell-impenetrable marine alkaloid that can suppress infection that is mediated by the envelope glycoprotein of human immunodeficiency virus type 1. We explored the antiviral action and mechanisms of this alkaloid against emerging enveloped RNA viruses that use endocytosis for infection. The alkaloid inhibited the infection of retroviral vectors that had been pseudotyped with the envelope glycoprotein of Ebola virus and SARS-CoV-2. The antiviral effects of lamellarin were independent of the retrovirus Gag-Pol proteins. Interestingly, although heparin and dextran sulfate suppressed the cell attachment of vector particles, lamellarin did not. In silico structural analyses of the trimeric glycoprotein of the Ebola virus disclosed that the principal lamellarin-binding site is confined to a previously unappreciated cavity near the NPC1-binding site and fusion loop, whereas those for heparin and dextran sulfate were dispersed across the attachment and fusion subunits of the glycoproteins. Notably, lamellarin binding to this cavity was augmented under conditions where the pH was 5.0. These results suggest that the final action of the alkaloid against Ebola virus is specific to events following endocytosis, possibly during conformational glycoprotein changes in the acidic environment of endosomes. Our findings highlight the unique biological and physicochemical features of lamellarin α20-sulfate and should lead to the further use of broadly reactive antivirals to explore the structural mechanisms of virus replication. ","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Viruses, 14(4), art. no. 816; 2022","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/v14040816","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"19994915","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Izumida, Mai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kotani, Osamu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayashi, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Smith, Chris"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuda, Tsutomu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suga, Koushirou"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwao, Masatomo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishibashi, Fumito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Hironori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kubo, Yoshinao"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-09"}],"displaytype":"detail","filename":"viruses14_816.pdf","filesize":[{"value":"4.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"viruses14_816.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27357/files/viruses14_816.pdf"},"version_id":"325b6915-fe47-4282-b0e2-e3a9df28a158"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"marine alkaloid","subitem_subject_scheme":"Other"},{"subitem_subject":"lamellarin α20-sulfate","subitem_subject_scheme":"Other"},{"subitem_subject":"emerging viruses","subitem_subject_scheme":"Other"},{"subitem_subject":"antiviral action","subitem_subject_scheme":"Other"},{"subitem_subject":"antiviral mechanism","subitem_subject_scheme":"Other"},{"subitem_subject":"pseudotyped lentiviral vector","subitem_subject_scheme":"Other"},{"subitem_subject":"molecular dynamics simulation","subitem_subject_scheme":"Other"},{"subitem_subject":"docking simulation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Unique Mode of Antiviral Action of a Marine Alkaloid against Ebola Virus and SARS-CoV-2","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Unique Mode of Antiviral Action of a Marine Alkaloid against Ebola Virus and SARS-CoV-2"}]},"item_type_id":"2","owner":"6","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-09"},"publish_date":"2022-05-09","publish_status":"0","recid":"27357","relation_version_is_last":true,"title":["Unique Mode of Antiviral Action of a Marine Alkaloid against Ebola Virus and SARS-CoV-2"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T19:50:25.719358+00:00"}