{"created":"2023-05-15T16:49:46.382911+00:00","id":27367,"links":{},"metadata":{"_buckets":{"deposit":"890a7628-40f6-45b8-8128-cb6ee5fae302"},"_deposit":{"created_by":6,"id":"27367","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27367"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027367","sets":["10:22:24"]},"author_link":["121755","121754"],"item_5_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"実験モデルにおける細胞構造体（バイオプラグ）を用いた肺切除後気管支断端瘻閉鎖に対する新たな治療法の確立"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"667","bibliographicPageStart":"660","bibliographicVolumeNumber":"34","bibliographic_titles":[{"bibliographic_title":"Interactive CardioVascular and Thoracic Surgery"}]}]},"item_5_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-02"}]},"item_5_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Nagasaki University (長崎大学)"}]}]},"item_5_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"OBJECTIVES: Cell therapies, such as stem cell suspension injection, are used to treat bronchopleural fistula. Although it is safe and effective, injected cells cannot remain within the bronchioles of the fistula due to cell leakage into the thoracic cavity. Here, we inserted a ‘bio plug’ into the fistula, produced using cells and a bio-3D printer, to examine the effectiveness of bio plugs for the closure of bronchopleural fistulas, the optimal cell source and the closure mechanism. METHODS: Bio plugs were made with mesenchymal stem (stromal) cells derived from bone marrow (MSCBM), fibroblasts and rat lung micro-vessel endothelial cells using a bio-3D printer with different cell mixing ratios. Six groups, according to the presence or absence and the type of bio plugs, were compared. The plugs were inserted into the bronchi of F344 rats. The obstruction ratio and histological and immunohistochemical findings were evaluated. RESULTS: MSCBM+ rat lung micro-vessel endothelial cell group exhibited a higher obstruction ratio among all groups excluding the MSCBM group (P = 0.039). This group had fibrosis and CD31-positive cells and fewer CD68-positive cells than MSCBM and MSCBM+ fibroblast groups. CONCLUSIONS: Bio plugs with mixed cells, including stem cells, contribute to bronchial closure in the current experimental setting. Endothelial cells effectively maintain the structure in this model. Although bronchial closure for bronchopleural fistula could not be described as clinical conditions were not reproduced, we collected essential data on bronchial closure; however, further experiments are warranted.","subitem_description_type":"Abstract"}]},"item_5_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"長崎大学学位論文 学位記番号:博(医歯薬)甲第1406号 学位授与年月日:令和4年3月2日","subitem_description_type":"Other"},{"subitem_description":"Author: Masaaki Moriyama, Keitaro Matsumoto, Daisuke Taniguchi, Ryusuke Machino, Tomoshi Tsuchiya, Koichi Nakayama, Takeshi Nagayasu","subitem_description_type":"Other"},{"subitem_description":"Citation: Interactive CardioVascular and Thoracic Surgery, 34(4), pp. 660–667; 2022","subitem_description_type":"Other"}]},"item_5_description_69":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Nagasaki University (長崎大学), 博士(医学) (2022-03-02)","subitem_description_type":"Other"}]},"item_5_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"17301甲医歯薬第1406号"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"121755","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Moriyama, Masaaki"}]}]},"item_5_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_5_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/icvts/ivab306","subitem_relation_type_select":"DOI"}}]},"item_5_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10069/00041409","subitem_relation_type_select":"HDL"}}]},"item_5_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2021. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com"}]},"item_5_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1569-9285","subitem_source_identifier_type":"ISSN"}]},"item_5_text_67":{"attribute_name":"学位の種類","attribute_value_mlt":[{"subitem_text_value":"課程博士"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森山, 正章"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-11"}],"displaytype":"detail","filename":"ISYK1406_Moriyama.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ISYK1406_Moriyama.pdf","objectType":"summary","url":"https://nagasaki-u.repo.nii.ac.jp/record/27367/files/ISYK1406_Moriyama.pdf"},"version_id":"15813645-cf50-4a57-879d-834035942ee9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bronchopleural fistula","subitem_subject_scheme":"Other"},{"subitem_subject":"Bio plug","subitem_subject_scheme":"Other"},{"subitem_subject":"Bio-3D printer","subitem_subject_scheme":"Other"},{"subitem_subject":"Mesenchymal stem cells derived from bone marrow","subitem_subject_scheme":"Other"},{"subitem_subject":"Endothelial cells","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Successful use of bio plugs for delayed bronchial closure after pneumonectomy in experimental settings","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Successful use of bio plugs for delayed bronchial closure after pneumonectomy in experimental settings","subitem_title_language":"en"}]},"item_type_id":"5","owner":"6","path":["24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-11"},"publish_date":"2022-05-11","publish_status":"0","recid":"27367","relation_version_is_last":true,"title":["Successful use of bio plugs for delayed bronchial closure after pneumonectomy in experimental settings"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-08-23T04:54:21.668194+00:00"}