{"created":"2023-05-15T16:31:20.782364+00:00","id":2737,"links":{},"metadata":{"_buckets":{"deposit":"b0c22dca-1b6b-4570-91f2-ea27182aaf04"},"_deposit":{"created_by":2,"id":"2737","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"2737"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00002737","sets":["27:28"]},"author_link":["12616","12615","12614"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"81","bibliographicPageStart":"78","bibliographicVolumeNumber":"548","bibliographic_titles":[{"bibliographic_title":"Analytical Biochemistry"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bacterial dipeptidyl-peptidase (DPP) 7 liberates a dipeptide with a preference for aliphatic and aromatic penultimate residues from the N-terminus. Although synthetic substrates are useful for activity measurements, those currently used are problematic, because they are more efficiently degraded by DPP5. We here aimed to develop a potent and specific substrate and found that the kcat/Km value for Phe-Met-methylcoumaryl-7-amide (MCA) (41.40?±?0.83?μM?1?s?1) was highest compared to Met-Leu-, Leu-Leu-, and Phe-Leu-MCA (1.06?3.77?μM?1?s?1). Its hydrolyzing activity was abrogated in a Porphyromonas gingivalis dpp7-knockout strain. Conclusively, we propose Phe-Met-MCA as an ideal synthetic substrate for DPP7.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Analytical Biochemistry, 548, pp.78-81; 2018","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ab.2018.02.008","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2018 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00032697","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nemoto, Takayuki K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Toshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohara-Nemoto, Yuko"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"AnalBiochem548_78.pdf","filesize":[{"value":"227.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AnalBiochem548_78.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/2737/files/AnalBiochem548_78.pdf"},"version_id":"1ed59029-af74-4189-9b38-860a71c73738"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Dipeptidyl-peptidase 5","subitem_subject_scheme":"Other"},{"subitem_subject":"Dipeptidyl-peptidase 7","subitem_subject_scheme":"Other"},{"subitem_subject":"Periodontopathic bacteria","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas gingivalis","subitem_subject_scheme":"Other"},{"subitem_subject":"Substrate specificity","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Establishment of potent and specific synthetic substrate for dipeptidyl-peptidase 7","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Establishment of potent and specific synthetic substrate for dipeptidyl-peptidase 7"}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-05-01"},"publish_date":"2020-05-01","publish_status":"0","recid":"2737","relation_version_is_last":true,"title":["Establishment of potent and specific synthetic substrate for dipeptidyl-peptidase 7"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:41:56.106259+00:00"}