@article{oai:nagasaki-u.repo.nii.ac.jp:00027372,
 author = {Han, Chengyan and Kim, Hee-Jin and Lee, Jae-Seong and Sakakura, Yoshitaka and Hagiwara, Atsushi},
 journal = {Aquatic Toxicology},
 month = {Mar},
 note = {Iron (Fe), a trace metal in coastal waters has increased significantly due to anthropogenic ac33 tivities, however, few studies have examined its toxicity to marine organism reproduction and 34 associated mechanisms. We employed two marine rotifers, the temperate Brachionus plicatilis, 35 and tropical B. rotundiformis to investigate the toxicity of iron (FeSO4ꞏ7H2O) and its deleteri36 ous effects on reproductive features in females (sexual fecundity, abnormal resting eggs, and 37 swimming speed) and males (lifespan, swimming speed, and spermatozoa quality) under lethal 38 and sub-lethal exposure. The 24-h median lethal concentration (LC50) of iron was determined 39 as 0.9 and 1.7 μg/mL per ng of dry weight for B. plicatilis and B. rotundiformis, respectively. 40 During sub-lethal iron (20–75 μg/mL) exposure, higher iron (≥ 20 μg/mL for B. plicatilis and 41 ≥ 45 μg/mL for B. rotundiformis) induced rotifer sexual toxicity especially in normal resting 42 egg development and production. These were supported by the data of male shorter lifespan, 43 poor sperm vitality, and rotifer behavioral changes as the iron concentration increased. Iron 44 effects on swimming behavior, slower males and faster females, should reduce male/female 45 encounter rates associated with inactive fertilized egg (resting egg) production. Two rotifer 46 species exhibited different iron-response patterns in genetic and enzymatic activities including 47 iron homeostasis-maintaining related Fe-S protein, and oxidative/antioxidant related lipid pe48 roxidation product (MDA), superoxidase dismutase/SOD, catalase/CAT, and cytochrome P450 49 under acute iron exposure. Antioxidant activities were vulnerable in B. plicatilis but kept ac50 tivities in B. rotundiformis, which may attribute to their temperate and tropical habitat adapta51 tions., Aquatic Toxicology, 246, art. no. 106135; 2022},
 title = {Iron reproductive toxicity of marine rotifer sibling species: Adaptation to temperate and tropical habitats},
 volume = {246},
 year = {2022}
}