{"created":"2023-05-15T16:49:47.300129+00:00","id":27389,"links":{},"metadata":{"_buckets":{"deposit":"5b4425fc-eca0-409f-888c-05b68727215c"},"_deposit":{"created_by":6,"id":"27389","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27389"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027389","sets":["10:11"]},"author_link":["121835","121836","121838","121839","121840","121837"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"G456","bibliographicPageStart":"G446","bibliographicVolumeNumber":"322","bibliographic_titles":[{"bibliographic_title":"American journal of physiology-Gastrointestinal and liver physiology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Angiotensin receptor blockers have been reported to be beneficial to liver fibrosis, but the relevant molecular and cellular mechanisms remain unclear. We herein investigated whether low-dose angiotensin receptor blocker alleviated liver fibrosis through mechanotransduction regulation. Hydrostatic pressure-induced liver fibrosis model was established in mice by ligating partially the inferior vena cava, and then randomly received a very low dose of losartan (0.5 mg/kg) or placebo treatment for 8 weeks. We found that losartan administration interfered the expression of several mechanotransductive molecules, and effectively alleviated liver fibrosis. Using a commercial device, we further confirmed that ex vivo loading of hepatic stellate cells to 50 mmHg hydrostatic pressure for 24 h significantly upregulated RhoA, ROCK, AT1R, and p-MLC2, which was effectively attenuated by adding 10 nM losartan in medium. Our in vivo and ex vivo experimental data suggest that low-dose angiotensin receptor blockers may alleviate hydrostatic pressure-induced liver fibrosis by altering the mechanotransduction properties of hepatic stellate cells. NEW & NOTEWORTHY Our ex vivo and in vivo experiments clearly indicated that low-dose losartan alleviated liver fibrosis, likely by modulating the mechanotransduction properties of HSCs. Uncovering the biomechanical signaling pathway of ARB treatment on liver fibrosis will be helpful to develop novel molecular targeting therapy for liver diseases.\n","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"American journal of physiology-Gastrointestinal and liver physiology, 322(4), pp. G446-G456; 2022","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Physiological Society"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1152/ajpgi.00238.2021","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 the American Physiological Society."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01931857","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Huang, Zisheng"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Khalifa, Mahmoud Osman"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Li, Peilin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Huang, Yu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Gu, Weili"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Li, Tao-Sheng"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-01"}],"displaytype":"detail","filename":"APGLP322_446.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"APGLP322_446.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27389/files/APGLP322_446.pdf"},"version_id":"afab1edf-f023-4c60-b48d-a22d1dee1c31"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"angiotensin receptor blocker","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatic stellate cells","subitem_subject_scheme":"Other"},{"subitem_subject":"hydrostatic pressure","subitem_subject_scheme":"Other"},{"subitem_subject":"liver fibrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"mechanotransduction","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Angiotensin receptor blocker alleviates liver fibrosis by altering the mechanotransduction properties of hepatic stellate cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Angiotensin receptor blocker alleviates liver fibrosis by altering the mechanotransduction properties of hepatic stellate cells"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-17"},"publish_date":"2022-05-17","publish_status":"0","recid":"27389","relation_version_is_last":true,"title":["Angiotensin receptor blocker alleviates liver fibrosis by altering the mechanotransduction properties of hepatic stellate cells"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T19:47:14.227767+00:00"}