{"created":"2023-05-15T16:49:48.421280+00:00","id":27417,"links":{},"metadata":{"_buckets":{"deposit":"34793cca-5ba0-42ac-b937-b2199acc4162"},"_deposit":{"created_by":6,"id":"27417","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27417"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027417","sets":["10:22:24"]},"author_link":["121951"],"item_5_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"エボラウイルスGPは宿主の細胞骨格シグナル伝達因子を介して内皮細胞を活性化する"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"142","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Viruses"}]}]},"item_5_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-18"}]},"item_5_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Nagasaki University (長崎大学)"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17301","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ebola virus disease (EVD) is a lethal disease caused by the highly pathogenic Ebola virus (EBOV), and its major symptoms in severe cases include vascular leakage and hemorrhage. These symptoms are caused by abnormal activation and disruption of endothelial cells (ECs) whose mediators include EBOV glycoprotein (GP) without the need for viral replication. However, the detailed molecular mechanisms underlying virus–host interactions remain largely unknown. Here, we show that EBOV-like particles (VLPs) formed by GP, VP40, and NP activate ECs in a GP-dependent manner, as demonstrated by the upregulation of intercellular adhesion molecules-1 (ICAM-1) expression. VLPs-mediated ECs activation showed a different kinetic pattern from that of TNF-α-mediated activation and was associated with apoptotic ECs disruption. In contrast to TNF-α, VLPs induced ICAM-1 overexpression at late time points. Furthermore, screening of host cytoskeletal signaling inhibitors revealed that focal adhesion kinase inhibitors were found to be potent inhibitors of ICAM-1 expression mediated by both TNF-α and VLPs. Our results suggest that EBOV GP stimulates ECs to induce endothelial activation and dysfunction with the involvement of host cytoskeletal signaling factors, which represent potential therapeutic targets for EVD.","subitem_description_type":"Abstract"}]},"item_5_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"長崎大学学位論文 学位記番号:博(医歯薬)甲第1435号 学位授与年月日:令和4年3月18日","subitem_description_type":"Other"},{"subitem_description":"Author: Benedicte Mpia Moni, Yasuteru Sakurai and Jiro Yasuda","subitem_description_type":"Other"},{"subitem_description":"Citation: Viruses, 14(1), art.no. 142; 2022","subitem_description_type":"Other"}]},"item_5_description_69":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Nagasaki University (長崎大学), 博士(医学) (2022-03-18)","subitem_description_type":"Other"}]},"item_5_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医歯薬第1435号"}]},"item_5_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_5_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/v14010142","subitem_relation_type_select":"DOI"}}]},"item_5_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10069/00041438","subitem_relation_type_select":"HDL"}}]},"item_5_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_5_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1999-4915","subitem_source_identifier_type":"ISSN"}]},"item_5_text_67":{"attribute_name":"学位の種類","attribute_value_mlt":[{"subitem_text_value":"課程博士"}]},"item_5_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Moni , Mpia Benedicte"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-26"}],"displaytype":"detail","filename":"ISYK1435_Moni.pdf","filesize":[{"value":"9.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ISYK1435_Moni.pdf","objectType":"fulltext","url":"https://nagasaki-u.repo.nii.ac.jp/record/27417/files/ISYK1435_Moni.pdf"},"version_id":"2731d9ac-0169-4b6b-9bed-f381ba10eeca"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ebola GP","subitem_subject_scheme":"Other"},{"subitem_subject":"ICAM-1","subitem_subject_scheme":"Other"},{"subitem_subject":"virus–host interactions","subitem_subject_scheme":"Other"},{"subitem_subject":"cytoskeletal signaling","subitem_subject_scheme":"Other"},{"subitem_subject":"focal adhesion kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"TNF-α","subitem_subject_scheme":"Other"},{"subitem_subject":"host factor","subitem_subject_scheme":"Other"},{"subitem_subject":"antivirals","subitem_subject_scheme":"Other"},{"subitem_subject":"micropinocytosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Rho signaling pathway","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Ebola Virus GP Activates Endothelial Cells via Host Cytoskeletal Signaling Factors","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ebola Virus GP Activates Endothelial Cells via Host Cytoskeletal Signaling Factors","subitem_title_language":"en"}]},"item_type_id":"5","owner":"6","path":["24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-26"},"publish_date":"2022-05-26","publish_status":"0","recid":"27417","relation_version_is_last":true,"title":["Ebola Virus GP Activates Endothelial Cells via Host Cytoskeletal Signaling Factors"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-08-23T04:57:14.571950+00:00"}