{"created":"2023-05-15T16:49:50.337631+00:00","id":27458,"links":{},"metadata":{"_buckets":{"deposit":"3a7a9331-2321-409c-996b-1e10b9e2a124"},"_deposit":{"created_by":6,"id":"27458","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27458"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027458","sets":["10:11"]},"author_link":["122039","122032","122037","122030","122036","122031","122034","122033","122035","122038","122029"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-06-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"41","bibliographicPageStart":"34","bibliographicVolumeNumber":"348","bibliographic_titles":[{"bibliographic_title":"Journal of Controlled Release"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Messenger RNA (mRNA) medicine has become a new therapeutic approach owing to the progress in mRNA delivery technology, especially with lipid nanoparticles (LNP). However, mRNA encapsulated-LNP (mRNA-LNP) cannot spontaneously cross the blood-brain barrier (BBB) which prevents the expression of foreign proteins in the brain. Microbubble-assisted focused ultrasound (FUS) BBB opening is an emerging technology that can transiently enhance BBB permeability. In this study, FUS/microbubble-assisted BBB opening was investigated for the intravenous delivery of mRNA LNP to the brain. The intensity of FUS irradiation was optimized to 1.5 kW/cm2, at which BBB opening occurred efficiently without hemorrhage or edema. Exogenous protein (luciferase) expression by mRNA-LNP, specifically at the FUS-irradiated side of the brain, occurred only when FUS and microbubbles were applied. This exogenous protein expression was faster but shorter than that of plasmid DNA delivery. Furthermore, foreign protein expression was observed in the microglia, along with CD31-positive endothelial cells, whereas no expression was observed in astrocytes or neurons. These results support the addition of mRNA-LNP to the lineup of nanoparticles delivered by BBB opening. ","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Controlled Release, 348, pp. 34-41; 2022","subitem_description_type":"Other"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jconrel.2022.05.042","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01683659","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ogawa, Koki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Michiharu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hiu, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuo, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mizukami, Shusaku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Omata, Daiki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Ryo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maruyama, Kazuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukai, Hidefumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Shigeru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-06-01"}],"displaytype":"detail","filename":"JCR348_34.pdf","filesize":[{"value":"833.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JCR348_34.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27458/files/JCR348_34.pdf"},"version_id":"a6d13d28-3030-4530-a9d6-bb53aea86a94"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Blood-brain barrier (BBB)","subitem_subject_scheme":"Other"},{"subitem_subject":"Focused ultrasound (FUS)","subitem_subject_scheme":"Other"},{"subitem_subject":"Lipid nanoparticles (LNP)","subitem_subject_scheme":"Other"},{"subitem_subject":"Microbubble","subitem_subject_scheme":"Other"},{"subitem_subject":"messenger RNA (mRNA)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Focused ultrasound/microbubbles-assisted BBB opening enhances LNP-mediated mRNA delivery to brain","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Focused ultrasound/microbubbles-assisted BBB opening enhances LNP-mediated mRNA delivery to brain"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-06-17"},"publish_date":"2022-06-17","publish_status":"0","recid":"27458","relation_version_is_last":true,"title":["Focused ultrasound/microbubbles-assisted BBB opening enhances LNP-mediated mRNA delivery to brain"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T19:43:38.736893+00:00"}