WEKO3
アイテム
Leucine-Rich Repeats and Transmembrane Domain 2 Controls Protein Sorting in the Striatal Projection System and Its Deficiency Causes Disturbances in Motor Responses and Monoamine Dynamic
http://hdl.handle.net/10069/00041620
http://hdl.handle.net/10069/00041620dee03100-c295-4f79-9b42-38095c4357ea
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ISYK1456_Ichise.pdf (11.4 MB)
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| アイテムタイプ | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2022-07-15 | |||||||
| タイトル | ||||||||
| タイトル | Leucine-Rich Repeats and Transmembrane Domain 2 Controls Protein Sorting in the Striatal Projection System and Its Deficiency Causes Disturbances in Motor Responses and Monoamine Dynamic | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Lrtm2 | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | gene targeting | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | striatal projection neuron | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | behavioral abnormality | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | monoamine metabolism | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | protein sorting | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
市瀬, 実里
× 市瀬, 実里
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| 著者別名 | ||||||||
| 識別子Scheme | WEKO | |||||||
| 識別子 | 122147 | |||||||
| 姓名 | Ichise , Misato | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | The striatum is involved in action selection, and its disturbance can cause movement disorders. Here, we show that leucine-rich repeats and transmembrane domain 2 (Lrtm2) controls protein sorting in striatal projection systems, and its deficiency causes disturbances in monoamine dynamics and behavior. The Lrtm2 protein was broadly detected in the brain, but it was enhanced in the olfactory bulb and dorsal striatum. Immunostaining revealed a strong signal in striatal projection output, including GABAergic presynaptic boutons of the SNr. In subcellular fractionation, Lrtm2 was abundantly recovered in the synaptic plasma membrane fraction, synaptic vesicle fraction, and microsome fraction. Lrtm2 KO mice exhibited altered motor responses in both voluntary explorations and forced exercise. Dopamine metabolite content was decreased in the dorsal striatum and hypothalamus, and serotonin turnover increased in the dorsal striatum. The prefrontal cortex showed age-dependent changes in dopamine metabolites. The distribution of glutamate decarboxylase 67 (GAD67) protein and gamma-aminobutyric acid receptor type B receptor 1 (GABABR1) protein was altered in the dorsal striatum. In cultured neurons, wild-type Lrtm2 protein enhanced axon trafficking of GAD67-GFP and GABABR1-GFP whereas such activity was defective in sorting signal-abolished Lrtm2 mutant proteins. The topical expression of hemagglutinin-epitope-tag (HA)-Lrtm2 and a protein sorting signal abolished HALrtm2 mutant differentially affected GABABR1 protein distribution in the dorsal striatum. These results suggest that Lrtm2 is an essential component of striatal projection neurons, contributing to a better understanding of striatal pathophysiology. | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第1456号 学位授与年月日:令和4年6月1日 | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Author: Misato Ichise, Kazuto Sakoori, Kei-ichi Katayama, Naoko Morimura, Kazuyuki Yamada, Hiroki Ozawa, Hayato Matsunaga, Minoru Hatayama, Jun Aruga | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Citation: Frontiers in Molecular Neuroscience, 15, art. no. 856315; 2022 | |||||||
| 書誌情報 |
Frontiers in Molecular Neuroscience 巻 15, p. 856315, 発行日 2022-06-01 |
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| EISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1662-5099 | |||||||
| DOI | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.3389/fnmol.2022.856315 | |||||||
| 権利 | ||||||||
| 権利情報 | © 2022 Ichise, Sakoori, Katayama, Morimura, Yamada, Ozawa, Matsunaga, Hatayama and Aruga. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| その他のタイトル | ||||||||
| その他のタイトル | Lrtm2は線条体投射システムにおけるタンパク質の選択的な輸送を制御し、その欠乏は運動機能とモノアミン動態の異常を引き起こす | |||||||
| 出版者 | ||||||||
| 出版者 | Frontiers Media S.A. | |||||||
| 関係URI | ||||||||
| 識別子タイプ | HDL | |||||||
| 関連識別子 | http://hdl.handle.net/10069/00041586 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士(医学) | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 17301 | |||||||
| 学位授与機関名 | Nagasaki University (長崎大学) | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2022-06-01 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲医歯薬第1456号 | |||||||
| 学位の種類 | ||||||||
| 値 | 課程博士 | |||||||
| 引用 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Nagasaki University (長崎大学), 博士(医学) (2022-06-01) | |||||||
