{"created":"2023-05-15T16:49:51.840367+00:00","id":27493,"links":{},"metadata":{"_buckets":{"deposit":"004a57f1-e34d-4403-9507-9a515853eb58"},"_deposit":{"created_by":6,"id":"27493","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27493"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027493","sets":["10:11"]},"author_link":["122190","122192","122189","122193","122187","122191","122188"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-06-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageStart":"6619","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Osteoclasts are multinucleated bone-resorbing cells that are formed by the fusion of macrophages. Recently, we identified Rab44, a large Rab GTPase, as an upregulated gene during osteoclast differentiation that negatively regulates osteoclast differentiation. However, the molecular mechanisms by which Rab44 negatively regulates osteoclast differentiation remain unknown. Here, we found that the GDP form of Rab44 interacted with the actin-binding protein, Coronin1C, in murine macrophages. Immunoprecipitation experiments revealed that the interaction of Rab44 and Coronin1C occurred in wild-type and a dominant-negative (DN) mutant of Rab44, but not in a constitutively active (CA) mutant of Rab44. Consistent with these findings, the expression of the CA mutant inhibited osteoclast differentiation, whereas that of the DN mutant enhanced this differentiation. Using a phase-contrast microscope, Coronin1C-knockdown osteoclasts apparently impaired multinuclear formation. Moreover, Coronin1C knockdown impaired the migration and chemotaxis of RAW-D macrophages. An in vivo experimental system demonstrated that Coronin1C knockdown suppresses osteoclastogenesis. Therefore, the decreased cell formation and fusion of Coronin1C-depleted osteoclasts might be due to the decreased migration of Coronin1C-knockdown macrophages. These results indicate that Coronin1C is a GDP-specific Rab44 effector that controls osteoclast formation by regulating cell motility in macrophages.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Molecular Sciences, 23 (12), art. no. 6619; 2022　","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms23126619","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamaguchi, Yu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kadowaki, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aibara, Nozomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohyama, Kaname"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, Kuniaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakai, Eiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsukuba, Takayuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-15"}],"displaytype":"detail","filename":"IJMS23_6619.pdf","filesize":[{"value":"3.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJMS23_6619.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27493/files/IJMS23_6619.pdf"},"version_id":"3c7a5bd8-9e9b-455d-8e12-b7b8c5c0a5d9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Rab44","subitem_subject_scheme":"Other"},{"subitem_subject":"Coronin1C","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoclast differentiation","subitem_subject_scheme":"Other"},{"subitem_subject":"effector","subitem_subject_scheme":"Other"},{"subitem_subject":"cell motility","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Coronin1C Is a GDP-Specific Rab44 Effector That Controls Osteoclast Formation by Regulating Cell Motility in Macrophages","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Coronin1C Is a GDP-Specific Rab44 Effector That Controls Osteoclast Formation by Regulating Cell Motility in Macrophages"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-07-15"},"publish_date":"2022-07-15","publish_status":"0","recid":"27493","relation_version_is_last":true,"title":["Coronin1C Is a GDP-Specific Rab44 Effector That Controls Osteoclast Formation by Regulating Cell Motility in Macrophages"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T19:41:47.883554+00:00"}