@article{oai:nagasaki-u.repo.nii.ac.jp:00027510,
 author = {神徳, 備子 and 江藤, 宏美},
 journal = {保健学研究, Health Science Research},
 month = {Jul},
 note = {目的：会陰裂傷予防方法の一つである会陰部温罨法について，その効果の指標として温熱刺激で誘導されるHSP70値をバイオマーカーに用いることが可能であるかを検証することを目的とした．
方法：20 ～ 39歳の健康女性10名を対象とし，一群で非介入期間と介入期間を設定した前後比較研究とした．22日間のうち最初 6 日目までをベースラインを設定するための非介入期間，非介入 6 日目の採血後より介入を開始し（介入 0 日目），16日目までを介入期間とし 3 日毎に計 6 回，最大温度40℃の温熱シートで40分間会陰部を加温した．血清HSP70値は非介入・介入期間に各 2 回ずつ採血しELISA法により定量した．
結果：血清HSP70値は，介入前 1 日目と 6 日目の間に有意な差を認めなかった（p = 0.24）．ベースラインである介入前 6 日目と介入後 7 日目及び16日目との間にもそれぞれ有意な差は認めなかった（p = 0.37, p = 0.07）．
結論：本研究では会陰部温罨法と血清HSP70値との間に有意な関連は認められなかった．これは，会陰部温罨法によってHSP70が血清には反映されなかった可能性が考えられる．現在，局所温罨法が当該部位の細胞にどのような影響を与えるのかは明らかになっていない．会陰裂傷予防に用いられる温罨法のメカニズムを明らかにする上で，HSPとの関連について検討していくことは重要であろう．
Objective: Perineal laceration is a frequent occurrence in vaginal deliveries, and it has been shown that the perineal warming methods are effective as a preventive method. In this study, we focused on heat shock protein (HSP) which promotes the prevention of cell damage and a decreased chance of perineal laceration. The purpose of this study was to examine whether the levels of HSP70 in serum in healthy female participants change upon the use of a perineal warm compress method and to verify whether HSP70 can be used as a biomarker to indicate the effectiveness of a perineal warm compress.
Methods: This study used a pre-post design with a group of participants who received no intervention for a period of time before receiving intervention for a perineal laceration. The participants were 10 healthy women aged 20-39 years. The study period was 22 days; the first 6 days was the non-intervention period, and the baseline of the levels of HSP70 in serum was measured on day 1 and day 6. After the first 6 days, there was the intervention period. The levels of HSP70 in serum were measured on day 7 and day 16 after the start of the intervention. A hot compress method uses a steam thermal sheet at a maximum temperature of 40°C; this is applied for 40 min at one time, every 3 days for a total of 6 times.
Results: The levels of HSP70 in serum were not significantly different between day 1 and day 6 of the non-intervention period (p = 0.24). There was no significant difference in the levels of HSP70 in serum between day 6 of the non-intervention period and on day 7 and day 16 of the intervention period (p = 0.37, p = 0.07, respectively).
Conclusions: There was no significant fluctuation in serum HSP70 levels before and after the use of a perineal hot compress method. It is possible that HSP70 was not reflected in the serum as a result of the use of the perineal hot compress method. Currently, little is known about how a localized hot compress method affects the perineal at a cellular level. Evaluating the association of HSP70 as a factor should be useful for clarifying the mechanism of a thermal method applied for perineal care during birth., 保健学研究, 35, pp.1-10; 2022},
 pages = {1--10},
 title = {健康成人女性を対象とした会陰部局所温罨法による血清HSP70の変化～会陰裂傷予防のためのパイロットスタディ～},
 volume = {35},
 year = {2022}
}