@article{oai:nagasaki-u.repo.nii.ac.jp:00027523,
 author = {岸川, 直哉 and 沼田, 翔 and 梅野, 智大 and EL-MAGHRABEY, Mahmoud and 大山, 要 and 田中, 正一 and 黒田, 直敬},
 issue = {6},
 journal = {分析化学, BUNSEKI KAGAKU},
 month = {Jun},
 note = {本研究では，非天然型アミノ酸である2-アミノイソ酪酸（2-aminoisobutyric acid, Aib）を含むペプチドで修飾した新規固定相を調製し，HPLCにおける分離挙動を調査した．1残基のAib及び2残基のL-ロイシンから構成されるトリペプチドであるAib 3mer，及びそれら2分子が縮合したヘキサペプチドであるAib 6merをaminopropylsilyl silica gel（APS）へと修飾後，ステンレススチール製カラムに充填した．調製したカラムについて，移動相中の有機溶媒含量の変化が低極性化合物の保持に与える影響を調査した．その結果，有機溶媒含量の増加に伴って保持が減少するという典型的な逆相モードの分離挙動が見られた．次に，移動相中の有機溶媒含量の変化が高極性化合物の保持に与える影響を調査した．その結果，移動相中の有機溶媒含量が高い領域において，有機溶媒含量の増加に伴う高極性化合物の保持時間が延長するHILICモードの分離挙動が確認された．本研究で開発した新規固定相は，低極性・高極性化合物の両方の分離に利用可能であることが示された．
In this study, a new stationary phase modified with a peptide containing 2-aminoisobutyric acid (Aib, an unnatural amino acid) was prepared, and its separation behavior in HPLC was investigated. Peptide Aib 3mer (L-Leu-L-Leu-Aib) and peptide Aib 6mer (L-Leu-L-Leu-Aib-L-Leu-L-Leu-Aib) were introduced into aminopropylsilyl silica gel (APS) and packed into a stainless-steel column. The effect of organic solvent content in the mobile phase on the retention of hydrophobic compounds was investigated for the prepared columns. The results showed a typical reversed-phase mode separation behavior, in which retention decreased with increasing organic solvent content. Next, the effect of organic solvent content in the mobile phase on the retention of hydrophilic compounds was investigated. As a result, the separation behavior in the region where the organic solvent content in the mobile phase is high follows the HILIC mode, where the retention of hydrophilic compounds increases with increasing organic solvent content. All of this demonstrates the versatility of the prepared new stationary phase and its efficiency in separating both hydrophobic and hydrophilic compounds., 分析化学, 71(6), pp. 351-356; 2022},
 pages = {351--356},
 title = {非天然型アミノ酸を含むペプチドにより修飾したHPLC固定相の調製とその評価},
 volume = {71},
 year = {2022}
}