{"created":"2023-05-15T16:49:53.129121+00:00","id":27524,"links":{},"metadata":{"_buckets":{"deposit":"01c37c40-a1bb-4551-9b22-0ba59847ce11"},"_deposit":{"created_by":6,"id":"27524","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27524"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027524","sets":["10:11"]},"author_link":["122341","122339","122340","122342"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-06-30","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"463275","bibliographicVolumeNumber":"1677","bibliographic_titles":[{"bibliographic_title":"Journal of Chromatography A"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A new fluorescence derivatization technique for the determination of the thyroid hormones, 3,3’,5-triiodo-L-thyronine (T3, triiodothyronine) and 3,3’,5,5’-tetraiodo-L-thyronine (T4, L-thyroxine), in human serum was developed based on the Sonogashira coupling reaction. This derivatization reaction was recently utilized by our research group as a promising solution for the derivatization of ortho-substituted aryl halides that suffer from steric hindrance. T3 and T4 possess amino groups that could be derivatized by many reagents; however, these reagents are not useful in the case of biological analysis as they could non-selectively react with many biogenic amines and amino acids. Thus, herein we aimed at labeling the iodo-phenyl group of T3 and T4 as a selective fluorescence labeling approach suitable for biological analysis. The fluorescent alkyne, 2-(4-ethynylphenyl)-4,5-diphenyl-1H-imidazole (DIB-ET), can label the ortho-substituted aryl halides T3 and T4 in the presence of palladium and copper as catalysts, overcoming the steric hindrance of ortho-substitution. Furthermore, the application of the proposed method for the selective analysis of T3 and T4 in biological samples was successfully performed even in the presence of numerous biological components.　The formed fluorescent derivatives produced from the reaction of DIB-ET and T3 and T4 could be determined by an HPLC system with fluorescence detection. The proposed method was successfully applied for the selective and sensitive determination of T3 and T4 in human serum with the detection limits (S/N = 3) of 4.0 and 6.1 ng/mL and the recovery rate in the range of 84.3-92.1% and 81.3-84.9%, respectively. Therefore, the proposed method could be used as a new simple tool for the simultaneous determination of T3 and T4 in biological samples.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Chromatography A, 1677, art. no. 463275; 2022","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.chroma.2022.463275","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00219673","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakano-Yasaka, Naoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kishikawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"El-Maghrabey, Mahmoud"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Naotaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-06-30"}],"displaytype":"detail","filename":"JCA1677_463275.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JCA1677_463275.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27524/files/JCA1677_463275.pdf"},"version_id":"545a754b-3807-48fc-aa82-6757df0ca4a3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fluorescence derivatization","subitem_subject_scheme":"Other"},{"subitem_subject":"Thyroid hormones","subitem_subject_scheme":"Other"},{"subitem_subject":"Sonogashira coupling reaction","subitem_subject_scheme":"Other"},{"subitem_subject":"Human serum","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluorescent alkyne","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of a selective fluorescence derivatization strategy for thyroid hormones based on the Sonogashira coupling reaction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of a selective fluorescence derivatization strategy for thyroid hormones based on the Sonogashira coupling reaction"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-08-05"},"publish_date":"2022-08-05","publish_status":"0","recid":"27524","relation_version_is_last":true,"title":["Development of a selective fluorescence derivatization strategy for thyroid hormones based on the Sonogashira coupling reaction"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T19:40:26.312280+00:00"}