{"created":"2023-05-15T16:49:55.061266+00:00","id":27569,"links":{},"metadata":{"_buckets":{"deposit":"bc5c04d6-2a41-42de-b99a-7062c5f4b4b7"},"_deposit":{"created_by":6,"id":"27569","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27569"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027569","sets":["10:11"]},"author_link":["122544","122537","122542","122545","122546","122548","122538","122543","122549","122547","122541","122540","122539"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-11-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"106480","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"International Journal of Antimicrobial Agents"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: The plasmid-mediated bacterial colistin-resistant gene, mcr, is of global concern in clini-cal healthcare. However, there are few reports of surveillance for mcr in Japan. The aim of this study was to assess the prevalence of colistin resistance by identifying nine mcr genes in extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae and carbapenem-resistant Enterobacteriaceae (CRE) isolates in Japan.\nMethods: A total of 273 ESBL and CRE clinical isolates were collected from patients in five tertiary hospi-tals from August 2016 to March 2017. Minimum inhibitory concentration (MIC) of colistin was measured using the microdilution method. Polymerase chain reaction (PCR) was performed to detect mcr-1 to mcr-9 genes in all strains. Whole-genome sequencing (WGS) analysis was conducted for any mcr-genes identi-fied that had not been previously reported in patients from Japan.\nResults: The rate of colistin resistance was 7.7% in all strains, with a higher rate in the CRE strains than in the ESBL-producing strains (20.4% versus 1.1%). The mcr-5 and mcr-9 gene were detected in one ESBL-producing Escherichia coli strain (1/273, 0.37%) and three CRE strains (3/273, 1.1%), respectively. As the\nESBL-producing E. coli strain was the first clinical strain with mcr-5 in Japan, WGS analysis was performed for the strain. The sequence type of the mcr-5-positive strain was ST1642 and it carried two distinct plasmids, ESBL gene-carrying pN-ES-6-1, and mcr-5.1-carrying pN-ES-6-2.\nConclusions: The results of this study showed that the frequency of colistin resistance and mcr-positive strains is not high in Japan. As the MIC for colistin was low in the mcr-5.1 and mcr-9 gene-positive strain, continuous monitoring of mcr genes is necessary.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Antimicrobial Agents, 59(1), art. no. 106480; 2022　","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Ltd"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ijantimicag.2021.106480","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09248579","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawamoto, Yasuhide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaku, Norihito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akamatsu, Norihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakamoto, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kosai, Kosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morinaga, Yoshitomo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohmagari, Norio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Izumikawa, Koichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Yoshihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mikamo, Hiroshige"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaku, Mitsuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oishi, Kazunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagihara, Katsunori"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-18"}],"displaytype":"detail","filename":"IJAA59_106480.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJAA59_106480.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27569/files/IJAA59_106480.pdf"},"version_id":"7d2146a9-1515-43a8-8ee9-551a02d1b11b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"mcr-5","subitem_subject_scheme":"Other"},{"subitem_subject":"mcr-9","subitem_subject_scheme":"Other"},{"subitem_subject":"colistin","subitem_subject_scheme":"Other"},{"subitem_subject":"Enterobacteriaceae","subitem_subject_scheme":"Other"},{"subitem_subject":"Surveillance","subitem_subject_scheme":"Other"},{"subitem_subject":"Japan","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The surveillance of colistin resistance and mobilized colistin resistance genes in multidrug-resistant Enterobacteriaceae isolated in Japan","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The surveillance of colistin resistance and mobilized colistin resistance genes in multidrug-resistant Enterobacteriaceae isolated in Japan"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-08-29"},"publish_date":"2022-08-29","publish_status":"0","recid":"27569","relation_version_is_last":true,"title":["The surveillance of colistin resistance and mobilized colistin resistance genes in multidrug-resistant Enterobacteriaceae isolated in Japan"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T19:38:30.781984+00:00"}