@article{oai:nagasaki-u.repo.nii.ac.jp:00002761,
 author = {朝重, 紅音 and 菅, 向志郎 and 金井, 欣也},
 journal = {長崎大学水産学部研究報告, Bulletin of the Faculty of Fisheries Nagasaki University},
 month = {Mar},
 note = {Quantitative PCR (qPCR) quantifies the template DNA from the number of threshold cycles (Ct value), but the probability of quantitative errors occurs due to the differences in recovery rates during 
DNA purification. In order to investigate the effect of different DNA purification kits in counting Edwardsiella tarda cells using qPCR, we compared two methods; the bacterial culture dilution method, in which the DNA is purified after 5 steps of a ten-fold serial dilution of E. tarda culture, and the DNA dilution method, which the DNA is diluted after purification with the optimum cell number of E. tarda. In both methods, we used three types of DNA purification kit: Chelex resin, Non-Silica membrane and Silica membrane. Our results showed the lowest Ct value and high DNA recovery rate were obtained using Chelex resin. Non-Silica membrane and Silica membrane Ct values in DNA dilution method were higher than that of Chelex resin. With the use of Non-Silica membrane, the calculated DNA using bacterial culture dilution method decreased by 67-87% compared to DNA dilution method at 1.0×10 4cells/μL of E. tarda., 長崎大学水産学部研究報告, 99, pp.7-11; 2018},
 pages = {7--11},
 title = {異なるDNA精製法が定量PCRによるEdwardsiella tardaの計数に与える影響},
 volume = {99},
 year = {2018}
}