@article{oai:nagasaki-u.repo.nii.ac.jp:00027633,
 author = {Nagahashi-Araki, Miyuki and Tasaka, Makoto and Takamura, Tsunehiko and Eto, Hiromi and Sasaki, Noriko and Fujita, Wakako and Miyazaki, Asuka and Morifuji, Kanako and Honda, Naoko and Miyamura, Tunetake and Nishitani, Shota},
 issue = {1},
 journal = {BMC Pregnancy and Childbirth},
 month = {Sep},
 note = {Background: Breastfeeding in the early postpartum period is expected to have mental benefits for mothers; however, the underlying sychobiological mechanisms remain unclear. Previously, we hypothesized that the release of oxytocin in response to the suckling stimuli during breastfeeding would mediate a calming effect on primiparous mothers, and we examined salivary oxytocin measurements in primiparous mothers at postpartum day 4 using saliva samples without extraction, which was erroneous. Thus, further confirmation of this hypothesis with a precise methodology was needed.
Methods: We collected saliva samples at three time points (baseline, feeding, and post-feeding) to measure oxytocin in 24 primiparous mothers on postpartum day 2 (PD2) and 4 (PD4) across the breastfeeding cycle. Salivary oxytocin levels using both extracted and unextracted methods were measured and compared to determine the qualitative differences. State and trait anxiety and clinical demographics were evaluated to determine their association with oxytocin changes.
Results: Breastfeeding elevated salivary oxytocin levels; however, it was not detected to a significant increase in the extraction method at PD4. We found a weak but significant positive correlation between changes in extracted and unextracted oxytocin levels during breastfeeding (feeding minus baseline); there were no other significant positive correlations. Therefore, we used the extracted measurement index for subsequent analysis. We showed that the greater the increase in oxytocin during breastfeeding, the lower the state anxiety, but not trait anxiety. Mothers who exclusively breastfed at the 1-month follow-up tended to be associated with slightly higher oxytocin change at PD2 than those who did not.
Conclusions: Breastfeeding in early postpartum days could be accompanied by the frequent release of oxytocin and lower state anxiety, potentially contributing to exclusive breastfeeding., BMC Pregnancy and Childbirth, 22 (1), art. no. 711; 2022},
 title = {Endogenous oxytocin levels in extracted saliva elevates during breastfeeding correlated with lower postpartum anxiety in primiparous mothers},
 volume = {22},
 year = {2022}
}