{"created":"2023-05-15T16:49:58.099169+00:00","id":27639,"links":{},"metadata":{"_buckets":{"deposit":"e3a0a41c-2049-4a78-8577-ff54dcdb4d72"},"_deposit":{"created_by":6,"id":"27639","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27639"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027639","sets":["10:11"]},"author_link":["122896","122900","122902","122905","122903","122904","122897","122901","122895","122898","122899","122906"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-09-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"18","bibliographicPageStart":"2816","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Cells"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Idiopathic pulmonary fibrosis is a chronic intractable lung disease, leading to　respiratory failure and death. Although anti-fibrotic agents delay disease progression, they are not considered curative treatments, and alternative modalities have attracted attention. We examined the effect of human γδ T cells on collagen type I in lung fibroblasts. Collagen type I was markedly reduced in a γδ T cell number-dependent manner following treatment with γδ T cells expanded with tetrakis-pivaloxymethyl 2-(thiazole-2-ylamino) ethylidene-1,1-bisphosphonate (PTA) and interleukin-2. Collagen type I levels remained unchanged on addition of γδ T cells to the culture system through a trans-well culture membrane, suggesting that cell–cell contact is essential for reducing its levels in lung fibroblasts. Re-stimulating γδ T cells with (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) reduced collagen type I levels without cell–cell contact, indicating the existence of HMBPP-induced soluble anti-fibrotic factors in γδ T cells. Adding anti-interferon-γ (IFN-γ)-neutralizing mAb restored collagen type I levels, demonstrating that human γδ T cell-derived IFN-γ reduces collagen type I levels. Conversely, interleukin-18 augmented γδ T cell-induced suppression of collagen type I. Therefore, human γδ T cells reduce collagen levels in lung fibroblasts via two distinct mechanisms; adoptive γδ T cell transfer is potentially a new therapeutic candidate.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Cells, 11 (18), art. no. 2816; 2022","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/cells11182816","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2073-4409","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okuno, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakamoto, Noriho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akiyama, Yoshiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tokito, Takatomo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hara, Atsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kido, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishimoto, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishimatsu, Yuji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tagod, Mohammed S. O."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamura, Haruki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Yoshimasa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukae, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-06"}],"displaytype":"detail","filename":"C11_2816.pdf","filesize":[{"value":"3.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"C11_2816.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27639/files/C11_2816.pdf"},"version_id":"db07009c-e81c-49e6-9cfe-79937a8aa719"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"γδ T cell","subitem_subject_scheme":"Other"},{"subitem_subject":"idiopathic pulmonary fibrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"HMBPP","subitem_subject_scheme":"Other"},{"subitem_subject":"interferon-γ","subitem_subject_scheme":"Other"},{"subitem_subject":"interleukin-18","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Two Distinct Mechanisms Underlying γδ T Cell-Mediated Regulation of Collagen Type I in Lung Fibroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Two Distinct Mechanisms Underlying γδ T Cell-Mediated Regulation of Collagen Type I in Lung Fibroblasts"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-06"},"publish_date":"2022-10-06","publish_status":"0","recid":"27639","relation_version_is_last":true,"title":["Two Distinct Mechanisms Underlying γδ T Cell-Mediated Regulation of Collagen Type I in Lung Fibroblasts"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T19:35:30.097849+00:00"}