{"created":"2023-05-15T16:49:58.403590+00:00","id":27646,"links":{},"metadata":{"_buckets":{"deposit":"a88cb3b9-d25a-4650-8309-ef1e664188f7"},"_deposit":{"created_by":6,"id":"27646","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27646"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027646","sets":["10:11"]},"author_link":["122932","122934","122937","122939","122931","122941","122935","122936","122938","122940","122933"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-09-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"1882","bibliographicPageStart":"1869","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"ACS Infectious Diseases"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Prion diseases are fatal neurodegenerative disorders caused by deposition of scrapie prion protein aggregates (PrPSc) in the brain. We previously reported that styrylchromone (SC) and benzofuran (BF) derivatives have potential as imaging probes for PrPSc. To further improve their properties, we designed and synthesized 2-(benzofuran-2-yl)-chromone (BFC) derivatives hybridized with SC and BF backbones as novel single-photon emission computed tomography probes for the detection of cerebral PrPSc deposits. Recombinant mouse prion protein (rMoPrP) aggregates and mouse-adapted bovine spongiform encephalopathy (mBSE)-infected mice were used to evaluate the binding properties of BFC derivatives to PrPSc. The BFC derivatives exhibited high binding affinities (equilibrium dissociation constant [Kd] = 22.6–47.7 nM) for rMoPrP aggregates. All BFC derivatives showed remarkable selectivity against amyloid beta aggregates. Fluorescence microscopy confirmed that the fluorescence signals of the BFC derivatives corresponded to the antibody-positive deposits of PrPSc in mBSE-infected mouse brains. Among the BFC derivatives, [125I]BFC-OMe and [125I]BFC-NH2 exhibited high brain uptake and favorable washout from the mouse brain. In vitro autoradiography demonstrated that the distribution of [125I]BFC-OMe in the brain tissues of mBSE-infected mice was co-localized with PrPSc deposits. Taken together, BFC derivatives appear to be promising prion imaging probes. ","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"ACS Infectious Diseases, 8(9), pp. 1869-1882; 2022","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Chemical Society"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1021/acsinfecdis.2c00142","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Infectious Diseases, copyright (c) American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsinfecdis.2c00142 "}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2373-8227","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakaie, Mari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Katayama, Fumihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagaki, Takehiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Sakura"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawasaki, Masao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishi, Kodai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogawa, Kazuma"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toriba, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishida, Noriyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakayama, Morio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fuchigami, Takeshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-09-09"}],"displaytype":"detail","filename":"ACSID8_1869.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ACSID8_1869.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27646/files/ACSID8_1869.pdf"},"version_id":"fe58618e-8c11-470e-92dc-3ce23a096e0a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Prion disease","subitem_subject_scheme":"Other"},{"subitem_subject":"PrPSc","subitem_subject_scheme":"Other"},{"subitem_subject":"amyloid","subitem_subject_scheme":"Other"},{"subitem_subject":"2-(benzofuran-2-yl)-chromone","subitem_subject_scheme":"Other"},{"subitem_subject":"single photon emission computed tomography (SPECT)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Synthesis and Biological Evaluation of Novel 2-(Benzofuran-2-yl)-chromone Derivatives for In Vivo Imaging of Prion Deposits in the Brain","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Synthesis and Biological Evaluation of Novel 2-(Benzofuran-2-yl)-chromone Derivatives for In Vivo Imaging of Prion Deposits in the Brain"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-13"},"publish_date":"2022-10-13","publish_status":"0","recid":"27646","relation_version_is_last":true,"title":["Synthesis and Biological Evaluation of Novel 2-(Benzofuran-2-yl)-chromone Derivatives for In Vivo Imaging of Prion Deposits in the Brain"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T19:35:10.460848+00:00"}