{"created":"2023-05-15T16:50:06.565701+00:00","id":27833,"links":{},"metadata":{"_buckets":{"deposit":"29639edf-35ac-41a7-9504-b0e4d85a403b"},"_deposit":{"created_by":6,"id":"27833","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27833"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027833","sets":["10:11"]},"author_link":["123962","123963","123968","123965","123966","123967","123969","123964"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"e12451","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"Reproductive Medicine and Biology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: Ovarian tissue cryopreservation and its auto-transplantation is promising technique in fertility preservation. Longevity of grafted tissue is limited though mechanism of follicle reduction is not fully understood. We evaluated histological alteration of vitrified-thawed ovarian tissue that grafted to nude mice. \nMaterials and Methods: Human ovarian tissue was cryopreserved by vitrification. After thawing, they were grafted to mesentery of nude mice. Twelve weeks after transplantation, the implants were removed and histologically examined. The presence of follicles, the degree of fibrosis, and TUNEL staining in surrounding cortex were evaluated. The stromal expressions of alpha-smooth muscle actin (aSMA) were determined.\nResults: Normal ovarian cortex was decreased, and fibrotic area were significantly increased after grafting. The distributions of developmental stage of follicles shifted toward activation of follicular growth. Stromal TUNEL staining was increased in frozen/thawed tissue. The expression of aSMA were found in perifollicular stroma of growing follicles, which were decreased in grafted tissue associated with reduction of cortical stroma.\nConclusions: Fibrosis, reduced cortical stroma, and activation of dormant follicles\nwere concomitantly observed in grafted ovarian cortex, which may relate to limited longevity. Perifollicular aSMA expression can be regarded as a marker of the competence of cortical stroma that regulate follicular development.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Reproductive Medicine and Biology, 21(1), art. no. e12451; 2022　","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley and Sons Ltd"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/rmb2.12451","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1445-5781","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kitajima, Michio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Murakami, Naoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitajima, Yuriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kajimura, Itsuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumura, Asako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumoto, Kanako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Harada, Ayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miura, Kiyonori"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-01-13"}],"displaytype":"detail","filename":"RMB21_e12451.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"RMB21_e12451.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27833/files/RMB21_e12451.pdf"},"version_id":"73402605-0b03-49c7-8ef7-76f482fcf1aa"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"alpha-smooth　muscle actin","subitem_subject_scheme":"Other"},{"subitem_subject":"fibrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"follicular growth","subitem_subject_scheme":"Other"},{"subitem_subject":"ovarian cortical stroma","subitem_subject_scheme":"Other"},{"subitem_subject":"ovarian tissue cryopreservation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice."}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-01-13"},"publish_date":"2023-01-13","publish_status":"0","recid":"27833","relation_version_is_last":true,"title":["Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice."],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T18:17:10.400994+00:00"}