@phdthesis{oai:nagasaki-u.repo.nii.ac.jp:00027900,
 author = {黄, 子圣},
 month = {Dec},
 note = {Angiotensin receptor blockers have been reported to be beneficial to liver　fibrosis, but the relevant molecular and cellular mechanisms remain unclear. We herein investigated whether low-dose angiotensin receptor blocker alleviated liver fibrosis through mechanotransduction regulation. Hydrostatic pressure-induced liver fibrosis model was established in mice by ligating partially the inferior vena cava, and then randomly received a very low dose of losartan (0.5 mg/kg) or placebo treatment for 8 weeks. We found that losartan administration interfered the expression of several mechanotransductive molecules, and effectively alleviated liver fibrosis. Using a commercial device, we further confirmed that ex vivo loading of hepatic stellate cells to 50 mmHg hydrostatic pressure for 24 h significantly upregulated RhoA, ROCK, AT1R, and p-MLC2, which was effectively attenuated by adding 10 nM losartan in medium. Our in vivo and ex vivo experimental data suggest that low-dose angiotensin receptor blockers may alleviate hydrostatic pressure-induced liver fibrosis by altering the mechanotransduction properties of hepatic stellate cells., 長崎大学学位論文 学位記番号:博(医歯薬)甲第1481号 学位授与年月日:令和4年12月7日, Author: Zisheng Huang, Mahmoud Osman Khalifa, Peilin Li, Yu Huang, Weili Gu, and Tao-Sheng Li, Citation: American Journal of  Physiology-Gastrointestinal and Liver Physiology, 322(4), pp. G446–G456; 2022, Nagasaki University (長崎大学), 博士(医学) (2022-12-07)},
 school = {Nagasaki University (長崎大学)},
 title = {Angiotensin receptor blocker alleviates liver fibrosis by altering the mechanotransduction properties of hepatic stellate cells},
 year = {2022}
}