{"created":"2023-05-15T16:50:10.434548+00:00","id":27921,"links":{},"metadata":{"_buckets":{"deposit":"c477f4ca-b907-4677-919c-1cb7437d28ff"},"_deposit":{"created_by":6,"id":"27921","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"27921"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00027921","sets":["10:11"]},"author_link":["124482","124481","124485","124488","124486","124480","124483","124484","124487"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-01-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"2173333","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Drug Delivery"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Liposomes are versatile carriers that can encapsulate various drugs; however, for delivery to the brain, they must be modified with a targeting ligand or other modifications to provide blood–brain barrier (BBB) permeability, while avoiding rapid clearance by reticuloendothelial systems through polyethylene glycol (PEG) modification. BBB-penetrating peptides act as brain-targeting ligands. In this study, to achieve efficient brain delivery of liposomes, we screened the functionality of eight BBB-penetrating peptides reported previously, based on high-throughput quantitative evaluation methods with in vitro BBB permeability evaluation system using Transwell, in situ brain perfusion system, and others. For apolipoprotein E mimetic tandem dimer peptide (ApoEdp), which showed the best brain-targeting and BBB permeability in the comparative evaluation of eight peptides, its lipid conjugate with serine–glycine (SG)5 spacer (ApoEdp-SG-lipid) was newly synthesized and ApoEdp-modified PEGylated liposomes were prepared. ApoEdp-modified PEGylated liposomes were effectively associated with human brain capillary endothelial cells via the ApoEdp sequence and permeated the membrane in an in vitro BBB model. Moreover, ApoEdp-modified PEGylated liposomes accumulated in the brain 3.9-fold higher than PEGylated liposomes in mice. In addition, the ability of ApoEdp-modified PEGylated liposomes to localize beyond the BBB into the brain parenchyma in mice was demonstrated via three-dimensional imaging with tissue clearing. These results suggest that ApoEdp-SG-lipid modification is an effective approach for endowing PEGylated liposomes with the brain-targeting ability and BBB permeability. ","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Drug Delivery, 30(1), art. no. 2173333; 2023","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Taylor and Francis Ltd."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1080/10717544.2023.2173333","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2023 The Author(s). Published by informa UK limited, trading as Taylor & Francis group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1071-7544","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kato, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamada, Sakura"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Rino"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iida, Yoshiki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumoto, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fumoto, Shintaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Arima, Hidetoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukai, Hidefumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Shigeru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-14"}],"displaytype":"detail","filename":"DD30_2173333.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DD30_2173333.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/27921/files/DD30_2173333.pdf"},"version_id":"e34afecf-df34-412f-bd46-82a0ee2d6aa3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Apolipoprotein E mimetic","subitem_subject_scheme":"Other"},{"subitem_subject":"peptide","subitem_subject_scheme":"Other"},{"subitem_subject":"blood–brain barrier","subitem_subject_scheme":"Other"},{"subitem_subject":"brain-targeting","subitem_subject_scheme":"Other"},{"subitem_subject":"liposomes","subitem_subject_scheme":"Other"},{"subitem_subject":"tissue clearing","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of an apolipoprotein E mimetic peptide–lipid conjugate for efficient brain delivery of liposomes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of an apolipoprotein E mimetic peptide–lipid conjugate for efficient brain delivery of liposomes"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-14"},"publish_date":"2023-02-14","publish_status":"0","recid":"27921","relation_version_is_last":true,"title":["Development of an apolipoprotein E mimetic peptide–lipid conjugate for efficient brain delivery of liposomes"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T18:13:26.719962+00:00"}