{"created":"2023-05-15T16:50:13.828395+00:00","id":28002,"links":{},"metadata":{"_buckets":{"deposit":"f9c92dee-6a31-4e0d-b310-d30105174442"},"_deposit":{"created_by":6,"id":"28002","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"28002"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00028002","sets":["12:13"]},"author_link":["124852","124850","124853","124849","124851","124855","124856","124847","124854","124848"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"12","bibliographicVolumeNumber":"51","bibliographic_titles":[{"bibliographic_title":"Tropical Medicine and Health"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background:Current therapeutic agents, including nifurtimox and benznidazole, are not sufficiently effective in the chronic phase of Trypanosoma cruzi infection and are accompanied by various side effects. In this study, 120 kinds of extracts from medicinal herbs used for Kampo formulations and 94 kinds of compounds isolated from medicinal herbs for Kampo formulations were screened for anti-T. cruzi activity in vitro and in vivo.\nMethods:As an experimental method, a recombinant protozoan cloned strain expressing luciferase, namely Luc2-Tulahuen, was used in the experiments. The in vitro anti-T. cruzi activity on epimastigote, trypomastigote, and amastigote forms was assessed by measuring luminescence intensity after treatment with the Kampo extracts or compounds. In addition, the cytotoxicity of compounds was tested using mouse and human feeder cell lines. The in vivo anti-T. cruzi activity was measured by a murine acute infection model using intraperitoneal injection of trypomastigotes followed by live bioluminescence imaging.\nResults:As a result, three protoberberine-type alkaloids, namely coptisine chloride, dehydrocorydaline nitrate, and palmatine chloride, showed strong anti-T. cruzi activities with low cytotoxicity. The IC50 values of these compounds differed depending on the side chain, and the most effective compound, coptisine chloride, showed a significant effect in the acute infection model.\nConclusions:For these reasons, coptisine chloride is a hit compound that can be a potential candidate for anti-Chagas disease drugs. In addition, it was expected that there would be room for further improvement by modifying the side chains of the basic skeleton.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Tropical Medicine and Health, 51(1), art. no. 12; 2023　","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BMC"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s41182-023-00502-2","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1349-4147","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tayama, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mizukami, Shusaku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toume, Kazufumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Komatsu, Katsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagi, Tetsuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nara, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tieu, Paul"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Huy, Nguyen Tien"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hamano, Shinjiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirayama, Kenji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-24"}],"displaytype":"detail","filename":"TMH51_12.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"TMH51_12.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/28002/files/TMH51_12.pdf"},"version_id":"9e9a2e4a-0111-47fe-92d0-58f7226d6001"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Trypanosoma cruzi","subitem_subject_scheme":"Other"},{"subitem_subject":"Chagas disease","subitem_subject_scheme":"Other"},{"subitem_subject":"Coptis rhizome extract","subitem_subject_scheme":"Other"},{"subitem_subject":"Coptisine chloride","subitem_subject_scheme":"Other"},{"subitem_subject":"Isoquinoline alkaloid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Anti-Trypanosoma cruzi activity of Coptis rhizome extract and its constituents","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Anti-Trypanosoma cruzi activity of Coptis rhizome extract and its constituents"}]},"item_type_id":"2","owner":"6","path":["13"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-03-24"},"publish_date":"2023-03-24","publish_status":"0","recid":"28002","relation_version_is_last":true,"title":["Anti-Trypanosoma cruzi activity of Coptis rhizome extract and its constituents"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-05-15T18:10:11.601629+00:00"}