@article{oai:nagasaki-u.repo.nii.ac.jp:00028008,
 author = {Hori, Yuma and Watanabe, Kaori and Yassen, Asmaa S. A. and Shirotani, Keiro and Tanaka, Takashi and Iwata, Nobuhisa},
 issue = {3},
 journal = {Biological and Pharmaceutical Bulletin},
 month = {Mar},
 note = {The onset of Alzheimer’s disease (AD) is characterized by accumulation of amyloid β peptide (Aβ) in the brain. Neprilysin (NEP) is one of the major Aβ-degrading enzymes. Given findings that NEP expression in the brain declines from the early stage of AD before apparent neuronal losses are observed, enhancement of NEP activity and expression may be a preventive and therapeutic strategy relevant to disease onset. We screened for compounds that could enhance the activity and expression of NEP using a polyphenol library previously constructed by our research group and investigated the structure–activity relationships of the identified polyphenols. We found that amentoflavone, apigenin, kaempferol, and chrysin enhanced the activity and expression of NEP, suggesting that chemical structures involving a double bond between positions 2 and 3 in the C ring of flavones are important for NEP enhancement, while catechol or pyrogallol structures, except for the galloyl group of catechins, abolished these effects. Moreover, natural compounds, such as quercetin, were not effective per se, but were changed to effective compounds by adding a lipophilic moiety. Using our study findings, we propose improvements for dietary habits with experimental evidence, and provide a basis for the development of novel small molecules as disease-modifying drugs for AD., Biological & pharmaceutical bulletin, 46(3), pp. 446-454; 2023},
 pages = {446--454},
 title = {Enhancement of Neprilysin Activity by Natural Polyphenolic Compounds and Their Derivatives in Cultured Neuroglioma Cells},
 volume = {46},
 year = {2023}
}