{"created":"2023-05-15T16:31:35.224940+00:00","id":3071,"links":{},"metadata":{"_buckets":{"deposit":"672a7645-80e3-4b1f-bb8d-d0f6f73c138a"},"_deposit":{"created_by":6,"id":"3071","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"3071"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00003071","sets":["10:22:24"]},"author_link":["115210","115209"],"item_5_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"マルチプレックス法を用いたアフリカトリパノソーマ症の血清診断法の開発"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-02-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"127","bibliographicPageStart":"121","bibliographicVolumeNumber":"65","bibliographic_titles":[{"bibliographic_title":"Parasitology International"}]}]},"item_5_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-02-03"}]},"item_5_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Nagasaki University (長崎大学)"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17301","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Human African trypanosomiasis (HAT) is a disease caused by Kinetoplastid infection. Serological tests are useful for epidemiological surveillance. The aim of this study was to develop a multiplex serological assay for HAT to assess the diagnostic value of selected HAT antigens for sero-epidemiological surveillance.\nWe cloned loci encoding eight antigens from Trypanosoma brucei gambiense, expressed the genes in bacterial systems, and purified the resulting proteins. Antigens were subjected to Luminex multiplex assays using sera from HAT and VL patients to assess the antigens' immunodiagnostic potential. Among T. b. gambiense antigens, the 64-kDa and 65-kDa invariant surface glycoproteins (ISGs) and flagellar calcium binding protein (FCaBP) had high sensitivity for sera from T. b. gambiense patients, yielding AUC values of 0.871, 0.737 and 0.858 respectively in receiver operating characteristics (ROC) analysis. The ISG64, ISG65, and FCaBP antigens were partially cross-reactive to sera from Trypanosoma brucei rhodesiense \npatients. The GM6 antigen was cross-reactive to sera from T. b. rhodesiense patients as well as to sera from VL patients. Furthermore, heterogeneous antibody responses to each individual HAT antigen were observed. Testing for multiple HAT antigens in the same panel allowed specific and sensitive detection. Our results demonstrate the utility of applying multiplex assays for development and evaluation of HAT antigens for use in sero-epidemiological surveillance.","subitem_description_type":"Abstract"}]},"item_5_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"長崎大学学位論文 学位記番号:博(医歯薬)乙第43号 学位授与年月日:平成28年2月3日","subitem_description_type":"Other"},{"subitem_description":"Author: Samson Muuo Nzou, Yoshito Fujii, Masashi Miura, Matilu Mwau, Anne Wanjiru Mwangi, Makoto Itoh, Md. Abdus Salam, Shinjiro Hamano, Kenji Hirayama, Satoshi Kaneko","subitem_description_type":"Other"},{"subitem_description":"Citation: Parasitology International, 65(2), pp.121-127; 2016","subitem_description_type":"Other"}]},"item_5_description_69":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Nagasaki University (長崎大学), 博士(医学) (2016-02-03)","subitem_description_type":"Other"}]},"item_5_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙医歯薬第43号"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"115210","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ンゾウ, サムソン　ムウオ"}]}]},"item_5_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_5_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.parint.2015.10.008","subitem_relation_type_select":"DOI"}}]},"item_5_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10069/36277","subitem_relation_type_select":"HDL"}}]},"item_5_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2015 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)."}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1383-5769","subitem_source_identifier_type":"ISSN"}]},"item_5_text_67":{"attribute_name":"学位の種類","attribute_value_mlt":[{"subitem_text_value":"論文博士"}]},"item_5_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nzou, Samson Muuo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-02-01"}],"displaytype":"detail","filename":"ISYO43_Nzou.pdf","filesize":[{"value":"852.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ISYO43_Nzou.pdf","objectType":"fulltext","url":"https://nagasaki-u.repo.nii.ac.jp/record/3071/files/ISYO43_Nzou.pdf"},"version_id":"24caadeb-7ecc-49ad-b911-f839310df793"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Human African trypanosomiasis","subitem_subject_scheme":"Other"},{"subitem_subject":"Sero-diagnosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Multiplex assay","subitem_subject_scheme":"Other"},{"subitem_subject":"Recombinant antigens","subitem_subject_scheme":"Other"},{"subitem_subject":"Sero-surveillance","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Development of multiplex serological assay for the detection of human African trypanosomiasis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of multiplex serological assay for the detection of human African trypanosomiasis","subitem_title_language":"en"}]},"item_type_id":"5","owner":"6","path":["24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-03-16"},"publish_date":"2016-03-16","publish_status":"0","recid":"3071","relation_version_is_last":true,"title":["Development of multiplex serological assay for the detection of human African trypanosomiasis"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-08-23T04:58:55.828902+00:00"}