@article{oai:nagasaki-u.repo.nii.ac.jp:00003203,
 author = {Fumoto, Shintaro and Taniguchi, Naoki and Ikai, Yuri and Yoshikawa, Naoki and Miyamoto, Hirotaka and Sasaki, Hitoshi and Hashida, Mitsuru and Kawakami, Shigeru and Nishida, Koyo},
 issue = {1},
 journal = {Gene and Cell Therapy},
 month = {Apr},
 note = {In the present study, we demonstrated the importance of PEGylation timing of galactosylated liposome/plasmid DNA (pDNA) complexes (lipoplexes) for liver-selective transfection in mice. Because a fenestrated endothelium can be a barrier for penetration of lipoplexes though sinusoids, the particle size of lipoplexes is one of the determining factors for in vivo liver parenchymal cell (hepatocyte, PC)-selective transfection. Here, we found that syn-insertion, as a novel PEGylation timing, was useful to control the particle size of galactosylated lipoplexes. Syn-insertion of PEGylated lipids was performed by simple mixing of pDNA solution containing PEGylated lipids and dispersion of the cationic liposomes. Both syn- and pre-insertion of PEGylated lipids decreased the particle size of lipoplexes, whereas post-insertion did not. Moreover, syn-insertion of PEGylated lipids to galactosylated lipoplexes improved liver selectivity and the PC/non-parenchymal cell ratio of transgene expression after intravenous injection in mice. Hence, these data will be valuable for the design and preparation of PEGylated lipoplexes for gene targeting., Gene and Cell Therapy, 1(1), pp.1-11; 2016},
 pages = {1--11},
 title = {The Insertion Timing of PEGylated Lipids to Galactosylated Lipoplexes Is Important for Liver-Selective Transfection in Mice},
 volume = {1},
 year = {2016}
}