{"created":"2023-05-15T16:31:40.842179+00:00","id":3203,"links":{},"metadata":{"_buckets":{"deposit":"b427222c-7194-4029-854c-0e9b7869552a"},"_deposit":{"created_by":2,"id":"3203","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"3203"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00003203","sets":["10:11"]},"author_link":["14203","14207","14201","14208","14206","14204","14202","14205","14209"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"11","bibliographicPageStart":"1","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"Gene and Cell Therapy"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In the present study, we demonstrated the importance of PEGylation timing of galactosylated liposome/plasmid DNA (pDNA) complexes (lipoplexes) for liver-selective transfection in mice. Because a fenestrated endothelium can be a barrier for penetration of lipoplexes though sinusoids, the particle size of lipoplexes is one of the determining factors for in vivo liver parenchymal cell (hepatocyte, PC)-selective transfection. Here, we found that syn-insertion, as a novel PEGylation timing, was useful to control the particle size of galactosylated lipoplexes. Syn-insertion of PEGylated lipids was performed by simple mixing of pDNA solution containing PEGylated lipids and dispersion of the cationic liposomes. Both syn- and pre-insertion of PEGylated lipids decreased the particle size of lipoplexes, whereas post-insertion did not. Moreover, syn-insertion of PEGylated lipids to galactosylated lipoplexes improved liver selectivity and the PC/non-parenchymal cell ratio of transgene expression after intravenous injection in mice. Hence, these data will be valuable for the design and preparation of PEGylated lipoplexes for gene targeting.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Gene and Cell Therapy, 1(1), pp.1-11; 2016","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Science Publishing Group"}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2016 Shintaro Fumoto et al. This article is distributed under Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)."}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fumoto, Shintaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taniguchi, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikai, Yuri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshikawa, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyamoto, Hirotaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashida, Mitsuru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishida, Koyo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"GCT1_1.pdf","filesize":[{"value":"3.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"GCT1_1.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/3203/files/GCT1_1.pdf"},"version_id":"1d3f27d7-daf8-48c1-b752-4c9ceefd4d03"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Gene Therapy","subitem_subject_scheme":"Other"},{"subitem_subject":"PEGylation","subitem_subject_scheme":"Other"},{"subitem_subject":"Galactosylated Liposomes","subitem_subject_scheme":"Other"},{"subitem_subject":"Targeting","subitem_subject_scheme":"Other"},{"subitem_subject":"Hepatocyte","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Insertion Timing of PEGylated Lipids to Galactosylated Lipoplexes Is Important for Liver-Selective Transfection in Mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Insertion Timing of PEGylated Lipids to Galactosylated Lipoplexes Is Important for Liver-Selective Transfection in Mice"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-04-18"},"publish_date":"2016-04-18","publish_status":"0","recid":"3203","relation_version_is_last":true,"title":["The Insertion Timing of PEGylated Lipids to Galactosylated Lipoplexes Is Important for Liver-Selective Transfection in Mice"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:35:53.399691+00:00"}