{"created":"2023-05-15T16:29:33.459951+00:00","id":348,"links":{},"metadata":{"_buckets":{"deposit":"60cee0a4-5f0d-47d6-b845-9df843f6d330"},"_deposit":{"created_by":2,"id":"348","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"348"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000348","sets":["8:9"]},"author_link":["1673","1672","1676","1675","1671","1674"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-10-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"20","bibliographicPageStart":"5117","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Transforming growth factor-β (TGF-β) plays a key role in bone metastasis formation; we hypothesized the possible involvement of TGF-β in the induction of cancer stem cells (CSCs) in the bone microenvironment (micro-E), which may be responsible for chemo-resistance. Methods: Mouse mammary tumor cells were implanted under the dorsal skin flap over the calvaria and into a subcutaneous (subQ) lesions in female mice, generating tumors in the bone and subQ micro-Es. After implantation of the tumor cells, mice were treated with a TGF-β R1 kinase inhibitor (R1-Ki). Results: Treatment with R1-Ki decreased tumor volume and cell proliferation in the bone micro-E, but not in the subQ micro-E. R1-Ki treatment did not affect the induction of necrosis or apoptosis in either bone or subQ micro-E. The number of cells positive for the CSC markers, SOX2, and CD166 in the bone micro-E, were significantly higher than those in the subQ micro-E. R1-Ki treatment significantly decreased the number of CSC marker positive cells in the bone micro-E but not in the subQ micro-E. TGF-β activation of the MAPK/ERK and AKT pathways was the underlying mechanism of cell proliferation in the bone micro-E. BMP signaling did not play a role in cell proliferation in either micro-E. Conclusion: Our results indicated that the bone micro-E is a key niche for CSC generation, and TGF-β signaling has important roles in generating CSCs and tumor cell proliferation in the bone micro-E. Therefore, it is critically important to evaluate responses to chemotherapeutic agents on both cancer stem cells and proliferating tumor cells in different tumor microenvironments in vivo.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Molecular Sciences, 20(20), art.no.5117; 2019","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms20205117","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"16616596","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14220067","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Futakuchi, Mitsuru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lami, Kris"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tachibana, Yuri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Yukari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Furukawa, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuoka, Junya"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"IJMS20_5117.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJMS20_5117.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/348/files/IJMS20_5117.pdf"},"version_id":"c117e170-1caa-428d-8169-18c46049753b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bone metastasis","subitem_subject_scheme":"Other"},{"subitem_subject":"Bone microenvironment","subitem_subject_scheme":"Other"},{"subitem_subject":"Cancer stem cell","subitem_subject_scheme":"Other"},{"subitem_subject":"Mammary tumor","subitem_subject_scheme":"Other"},{"subitem_subject":"Non CSC","subitem_subject_scheme":"Other"},{"subitem_subject":"TGF-β","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Effects of TGF-β Signaling on Cancer Cells and Cancer Stem Cells in the Bone Microenvironment","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Effects of TGF-β Signaling on Cancer Cells and Cancer Stem Cells in the Bone Microenvironment"}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-01"},"publish_date":"2019-11-01","publish_status":"0","recid":"348","relation_version_is_last":true,"title":["The Effects of TGF-β Signaling on Cancer Cells and Cancer Stem Cells in the Bone Microenvironment"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:18:20.992523+00:00"}