{"created":"2023-05-15T16:29:33.712805+00:00","id":354,"links":{},"metadata":{"_buckets":{"deposit":"cfc0288f-07b0-46de-87bc-a7daf6997305"},"_deposit":{"created_by":2,"id":"354","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"354"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000354","sets":["10:11"]},"author_link":["1712","1711","1715","1714","1719","1713","1718","1716","1717","1720","1721","1722","1710"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-10-17","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"304","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Stem Cell Research & Therapy"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: There are currently no effective treatments available for patients with irreversible loss of salivary gland (SG) function caused by radiation therapy for head and neck cancer. In this study, we have developed an effective culture method to enhance the anti-inflammatory and vasculogenic phenotypes of peripheral blood mononuclear cells (PBMNCs) and investigated whether such effectively conditioned PBMNCs (E-MNCs) could regenerate radiation-injured SGs and ameliorate salivary secretory function in mice. Methods: Mouse PBMNCs were expanded in primary serum-free culture with five vasculogenic proteins for 5 days, and then the resulting cells (E-MNCs) were analyzed for their characteristics. Subsequently, 5 104 E-MNCs (labeled with EGFP in some experiments) were injected intra-glandularly into a mouse model of radiation-injured atrophic submandibular glands. After 2-3 weeks, the submandibular glands were harvested, and then the injected E-MNCs were tracked. Four, 8, and 12 weeks after irradiation (IR), salivary outputs were measured to evaluate the recovery of secretory function, and the gland tissues were harvested for histological and gene expression analyses to clarify the effects of cell transplantation. Results: The resulting E-MNCs contained an enriched population of definitive CD11b/CD206-positive (M2 macrophage-like) cells and showed anti-inflammatory and vasculogenic characteristics. Salivary secretory function in E-MNC-transplanted mice gradually recovered after 4 weeks post-irradiation (post-IR) and reached 3.8-fold higher than that of non-transplanted mice at 12 weeks. EGFP-expressing E-MNCs were detected in a portion of the vascular endothelium and perivascular gland tissues at 2 weeks post-IR, but mainly in some microvessels at 3 weeks. Between 4 and 12 weeks post-IR, mRNA expression and histological analyses revealed that E-MNC transplantation reduced the expression of inflammatory genes and increased the level of tissue-regenerative activities such as stem cell markers, cell proliferation, and blood vessel formation. At 12 weeks post-IR, the areas of acinar and ductal cells regenerated, and the glands had less fibrosis. Conclusions: This effective conditioning of PBMNCs is a simple, rapid, and efficient method that provides a non-invasive source of therapeutic cells for regenerating radiation-injured atrophic SGs.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Stem cell research & therapy, 10(1), art.no.304; 2019","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s13287-019-1414-7","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and　reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"17576512","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"I, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sumita, Yoshinori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Takako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Honma, Ryo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwatake, Mayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Raudales, Jorge Luis Montenegro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shizuno, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroshima, Shinichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuda, Haruchika"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seki, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tran, Simon D."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asahara, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asahina, Izumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"SCRT10_304.pdf","filesize":[{"value":"42.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SCRT10_304.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/354/files/SCRT10_304.pdf"},"version_id":"6001b0ba-4c98-4a9d-8aa4-33c6cee28ee4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Radiogenic salivary gland dysfunction","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell-based therapy","subitem_subject_scheme":"Other"},{"subitem_subject":"Peripheral blood mononuclear cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Macrophage","subitem_subject_scheme":"Other"},{"subitem_subject":"Anti-inflammation","subitem_subject_scheme":"Other"},{"subitem_subject":"Vasculogenesis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Anti-inflammatory and vasculogenic conditioning of peripheral blood mononuclear cells reinforces their therapeutic potential for radiation-injured salivary glands","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Anti-inflammatory and vasculogenic conditioning of peripheral blood mononuclear cells reinforces their therapeutic potential for radiation-injured salivary glands"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-06"},"publish_date":"2019-11-06","publish_status":"0","recid":"354","relation_version_is_last":true,"title":["Anti-inflammatory and vasculogenic conditioning of peripheral blood mononuclear cells reinforces their therapeutic potential for radiation-injured salivary glands"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:18:11.678580+00:00"}