{"created":"2023-05-15T16:32:03.861417+00:00","id":3737,"links":{},"metadata":{"_buckets":{"deposit":"50c48be2-5853-4291-bbd5-6f9fe4f27bfc"},"_deposit":{"created_by":2,"id":"3737","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"3737"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00003737","sets":["73:74"]},"author_link":["15243","15245","15241","15242","15244","15240"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"58","bibliographicPageStart":"52","bibliographicVolumeNumber":"64","bibliographic_titles":[{"bibliographic_title":"Chemical and Pharmaceutical Bulletin"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The intracellular metabolism of selenium in the brain currently remains unknown, although the antioxidant activity of this element is widely acknowledged to be important in maintaining brain functions. In this study, a comprehensive method for identifying the selenium-binding proteins using PenSSeSPen as a model of the selenium metabolite, selenotrisulfide (RSSeSR, STS), was applied to a complex cell lysate generated from the rat brain. Most of the selenium from L-penicillamine selenotrisulfide (PenSSeSPen) was captured by the cytosolic protein thiols in the form of STS through the thiol-exchange reaction (R-SH+PenSSeSPen→R-SSeSPen+PenSH). The cytosolic protein species, which reacted with the PenSSeSPen mainly had a molecular mass of less than 20 kDa. A thiol-containing protein at m/z 15155 in the brain cell lysate was identified as the cystatin-12 precursor (CST12) from a rat protein database search and a tryptic fragmentation experiment. CST12 belongs to the cysteine proteinase inhibitors of the cystatin superfamily that are of interest in mechanisms regulating the protein turnover and polypeptide production in the central nervous system and other tissues. Consequently, CST12 is suggested to be one of the cytosolic proteins responsible for the selenium metabolism in the brain.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Chemical and Pharmaceutical Bulletin, 64(1), pp.52-58; 2016","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/cpb.c15-00689","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2016 The Pharmaceutical Society of Japan"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00092363","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13475223","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Pharmaceutical Society of Japan"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yoshida, Sakura"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hori, Eriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ura, Sakiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Haratake, Mamoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fuchigami, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakayama, Morio"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"CPBul64_52.pdf","filesize":[{"value":"800.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CPBul64_52.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/3737/files/CPBul64_52.pdf"},"version_id":"d3162c60-22fb-4c11-b846-326a0c41b8d1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"selenium","subitem_subject_scheme":"Other"},{"subitem_subject":"selenotrisulfide","subitem_subject_scheme":"Other"},{"subitem_subject":"cystatin-12 precursor","subitem_subject_scheme":"Other"},{"subitem_subject":"mass spectrometry","subitem_subject_scheme":"Other"},{"subitem_subject":"thiol-exchange","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Comprehensive Analysis of Selenium-Binding Proteins in the Brain Using Its Reactive Metabolite","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Comprehensive Analysis of Selenium-Binding Proteins in the Brain Using Its Reactive Metabolite"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-11-01"},"publish_date":"2016-11-01","publish_status":"0","recid":"3737","relation_version_is_last":true,"title":["A Comprehensive Analysis of Selenium-Binding Proteins in the Brain Using Its Reactive Metabolite"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:29:23.865113+00:00"}