@article{oai:nagasaki-u.repo.nii.ac.jp:00003742,
 author = {Hagiwara, Atsushi and Kim, Hee-Jin and Matsumoto, Hokuto and Ohta, Yusuke and Morita, Tetsuro and Hatanaka, Akimasa and Ishizuka, Risa and Sakakura, Yoshitaka},
 issue = {5},
 journal = {Fisheries Science},
 month = {Sep},
 note = {We evaluated the effectiveness of two representative marine zooplankton, the harpacticoid copepod Tigriopus japonicus and the euryhaline cladoceran Diaphanosoma celebensis, as live food for red sea bream Pagrus major larvae. Chicken-dropping extract (CDE) was applied to both zooplankton cultures to improve population growth. Population growth of both zooplankton was significantly enhanced by CDE supplementation (at 1 or 2 ml/l). The highest amount of docosahexaenoic acid (DHA) and higher DHA/eicosapentaenoic acid ratio were detected in T. japonicus, whereas D. celebensis showed similar values to that of Artemia. Effectiveness of both animals as live food was tested by rearing red sea bream larvae on them for 28 days and comparing the results with those for Artemia. There were no significant differences in total length (8.6 ± 1.1?8.7 ± 0.7 mm) or wet weight (8.2 ± 0.3?9.4 ± 0.1 mg) among fish larvae feeding on the three different zooplankton. Survival rate was significantly higher with T. japonicus (39.4 ± 3.1 %) than with D. celebensis (20.8 ± 3.8 %) and Artemia (16.7 ± 9.8 %). Viability was significantly higher in fish fed with T. japonicus (60.0 ± 27.8 %) and D. celebensis (60.0 ± 32.2 %) than in those fed with Artemia (44.4 ± 12.3 %). Fish fed with T. japonicus contained higher n-3 highly unsaturated fatty acids than those fed with D. celebensis and Artemia. It is concluded that T. japonicus and D. celebensis have high potential as live food in marine fish larviculture., Fisheries Science, 82(5), pp.799?809; 2016},
 pages = {799--809},
 title = {Production and use of two marine zooplanktons, Tigriopus japonicus and Diaphanosoma celebensis, as live food for red sea bream Pagrus major larvae},
 volume = {82},
 year = {2016}
}